【摘要】 肽核酸(PNA)是一类以多肽骨架替代DNA的戊糖磷酸骨架的寡核苷酸类似物,基本的骨架是N-(2-氨基乙基)甘氨酸。肽核酸具有良好的理化性质和生物学特性,如稳定性高、杂交特异性强、不被核酸酶和蛋白酶水解等,但其水溶性差,细胞膜通透性低。笔者从骨架、碱基、骨架和碱基的连接方式与位置等方面对修饰性肽核酸的合成研究进展进行综述,使肽核酸具有更好的应用前景。更多还原

【Abstract】 Peptide nucleic acid( PNA) is DNA mimic,in which the sugar-phosphate backbone is replaced by polyamide structure.Its basic framework is N-( 2-aminoethyl) glycine. PNA has good chemical,physical,and biological properties,such as high stability,strong specificity,not hybridization by nuclease and protease. But it has poor water solubility,low membrane permeability. In this paper,the synthesis of modified peptide nucleic acid is reviewed from the backbone,bases and the linker of backbone and base,which makes PNA have better application prospect.更多还原