【摘要】 为将白僵菌应用于烟叶储存醇化的仓储环境,对其进行抗干旱、耐高温菌株选育。通过温度筛选获得能够在35℃下生长的球孢白僵菌GZUIFR-GL5菌株。该菌株通过紫外线和微波在150s下的组合诱变和PEG胁迫筛选,得到具有生长稳定且能够耐受-0.8Mpa的较低水势和35℃环境条件的突变菌株GZUIFR-GL5-08i。测定GZUIFR-GL5-08i部分生理指标发现蛋白含量、脯氨酸、SOD、CAT、POD等含量均比诱变前菌株提高,反映了在抗旱机理方面虫生真菌与植物抗逆机理有相似性。更多还原

【Abstract】 Screening and breeding of drought-resistant and high temperature-resistant Beauveria bassiana was conducted so as to make it adaptable in leaf tobacco warehouse. GZUIFR-GL5-08 i strains were screened out through a combined ultraviolet and microwave mutagenic experiment. Results showed that GZUIFR-GL5-08 i sustained stable growth on the condition of water potential 0.8Mpa and temperature 35 ℃. Content of protein, proiline, SOD, CAT, POD were higher than that before mutation, which showed similarity in draught-resistance mechanism between entomogenous fungi and plants.更多还原