【摘要】 目的:建立同时测定消核灵胶囊中延胡索乙素和迷迭香酸含量的方法。方法:采用高效液相色谱法。色谱柱为Phenomenex C18,流动相A为0.1%磷酸(以三乙胺调p H至6.0)-甲醇(45∶55,V/V),流动相B为0.1%磷酸-甲醇(55∶45,V/V),梯度洗脱,流速为0.8 ml/min,检测波长为282 nm(0~25 min)、330 nm(25.01~60 min),柱温为28℃,进样量为10μl。结果:延胡索乙素和迷迭香酸检测质量浓度分别为1.502~75.10、6.03~301.6μg/ml(r均为0.999 8);精密度、重复性、稳定性试验的RSD≤1.36%;加样回收率分别为97.11%~99.87%、98.47%~101.99%,RSD分别为0.95%、1.08%(n=9)。结论:该方法操作简便、快速易行,结果准确、可靠,可作为消核灵胶囊中延胡索乙素和迷迭香酸的含量测定方法。更多还原

【Abstract】 OBJECTIVE:To establish a method for the contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase A of 0.1% phosphoric acid(adjusted p H to 6.0 with triethylamine)-methanol(45∶55,V/V)and(adjusted p H to 6.0 with triethylamine)B of 0.1% phosphoric acid-methanol(55∶45,V/V)(gradient elution)at flow rate of 0.8 ml/min,detection wavelength was 282 nm(0-25 min)and330 nm(25.01-60 min)and volume size was 10 μl. RESULTS:The linear range was 1.502-75.10 μg/ml for tetrahydropalmatine and6.03-301.6 μg/ml for rosmarinic acid(r=0.999 8);RSDs of precision,reproducibility and stability tests were ≤1.36%;recoveries were 97.11-99.87%(RSD=0.95%,n=9)and 98.47-101.99%(RSD=1.08%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and reliable,and can be used for contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule.更多还原