【摘要】 目的:研究丹参酮ⅡA逆转多药耐药的体外效应。方法:以0[仅20 mg/L多柔比星(ADM)或顺铂(DDP),阴性对照]、5mg/ml丹参酮ⅡA(联合20 mg/L ADM或DDP)培养人乳腺癌耐ADM(MCF-7/ADM)细胞、人肺癌耐DDP(A549/DDP)细胞24、48 h后,采用MTT法测定细胞活力;聚合酶链反应(PCR)法测定细胞中细胞周期控制蛋白CDC25A、细胞周期蛋白依赖性激酶2(CKD2)m RNA表达。结果:与阴性对照比较,丹参酮ⅡA作用于MCF-7/ADM、A549/DDP细胞24、48 h后细胞活力减弱,细胞中CDC25A、CKD2 m RNA表达减弱,差异有统计学意义(P<0.01)。结论:丹参酮ⅡA联合ADM或DDP能够抑制MCF-7/ADM和A549/DDP的细胞活力,减弱细胞中CDC25A、CKD2 m RNA表达,具有一定的逆转恶性肿瘤多药耐药的作用。更多还原

【Abstract】 OBJECTIVE:To study in vitro effects of tanshinone ⅡAto reverse multiple drug resistance. METHODS:MCF-7/ADM cells and A549/DDP cells were cultured with 0 [20 mg/L doxorubicin(ADM)or cisplatin(DDP),negative control],5 mg/ml tanshinone ⅡA(combined with 20 mg/L ADM or DDP)for 24 and 48 h. Then MTT method was used to determine cell viability,and polymerase chain reaction(PCR)was adopted to detect m RNA expressions of cell cycle control protein CDC25 A and cell cyclin dependent kinase(CKD2). RESULTS:Compared to the negative control,after MCF-7/ADM cells and A549/DDP cells were cultured with tanshinone ⅡAfor 24 and 48 h,cell viability was weaker,also were m RNA expressions of CDC25 A and CKD2.There were statistical differences(P<0.01). CONCLUSIONS:Tanshinone ⅡAcombined with ADM or DDP can inhibit the viability of cell line MCF-7/ADM and cell line A549/DDP,decrease expression of CDC25 A,CKD2 m RNA in cells and reverse multiple drug resistance in malignant tumors.更多还原