【摘要】 目的:研究百癣夏塔热片对模型小鼠变应性接触性皮炎(ACD)的抑制作用。方法:60只BALB/c小鼠随机均分为正常对照(等容溶剂)组、模型(等容溶剂)组、依巴斯汀(阳性对照,0.003 g/kg)组与百癣夏塔热片高、中、低剂量组(2.0、1.0、0.5 g/kg),ig给药,每天1次,连续14 d。于给药前第1、2天在小鼠备皮部位涂含0.5%二硝基氟苯(DNFB)的丙酮橄榄油溶液致敏,于给药第16天在左耳涂含0.2%DNFB的丙酮橄榄油溶液以复制小鼠ACD模型。模型复制成功48 h后,测定小鼠左耳缘厚度并计算差值与肿胀度;流式细胞仪检测小鼠血液中T淋巴细胞亚群CD4+、CD8+水平并计算CD4+/CD8+值;检测小鼠血清中白细胞介素4(IL-4)、IL-6水平。结果:与正常对照组比较,模型组小鼠左耳缘厚度差增加,肿胀度增加,血液中CD4+水平、CD4+/CD8+值降低,血清IL-6含量增加,差异有统计学意义(P<0.01)。与模型组比较,百癣夏塔热片高、中、低剂量组小鼠左耳肿胀度减少,血液中CD4+水平升高;百癣夏塔热片高、中剂量组小鼠左耳缘厚度差减少,血清IL-6含量减少;百癣夏塔热片高剂量组小鼠血液中CD8+水平升高,差异有统计学意义(P<0.01或P<0.05)。结论:百癣夏塔热片对模型小鼠ACD有一定抑制作用,其机制可能与平衡血液中CD4+、CD8+水平,减少血清中IL-6含量有关。更多还原

【Abstract】 OBJECTIVE:To investigate the inhibitory effect of Baixuan xiatare tablet on the model mouse with allergic contact dermatitis(ACD).METHODS:60 BALB/c mice were equally randomized into normal control(isometric solvent)group,model(isometric solvent)group,ebastine(positive control,0.003 g/kg)group and the groups of high,middle and low doses of Baixuan xiatare tablet(2.0,1.0 and 0.5 g/kg).The mice were given drugs,ig,once daily for 14 consecutive days.0.5% 2,4-dinitrofluorobenzene(DNFB)acetone olive oil solution was applied,for sensitization,on the prepared mouse’s skins one and two days before administration,and 0.2% DNFB acetone olive oil solution on their left ears 16 days thereafter to establish mouse models of ACD.At 48 h after successful establishment of the models,the thickness of the mouse’s left ear margin was measured and the difference value and swelling degree were calculated;flow cytometer was used to determine the levels of T lymphocyte subsets CD4+and CD8+in mouse blood and calculate the ratio of CD4+to CD8+;the levels of interleukin 4(IL-4)and IL-6 in mouse serum were determined.RESULTS:Compared with normal control group,those in the model group had higher difference value of ear margin and swelling degree,lower level of CD4+in blood and ratio of CD4+to CD8+,and higher content of IL-6 in serum.There was statistically difference(P<0.01).Compared with model group,those in the groups of high,middle and low doses of Baixuan xiatare tablet had lower degree of left ear swelling and higher level of CD4+in blood;those in the groups of high and middle doses thereof had lower difference value of left ear margin and level of IL-6 in serum;and those in the group of high dose thereof had higher level of CD8+in blood.There was statistically significance(P<0.01 or P<0.05).CONCLUSIONS:Baixuan xiatare tablet has inhibitory effect to some degree on the mouse model with ACD by a mechanism which may be related to the balance of subsets CD4+and CD8+in blood and the reduction of IL-6 in serum.更多还原