【摘要】 目的:研究胰岛素自微乳剂肠道给药在1型糖尿病模型大鼠体内的降血糖效果。方法:取大鼠ip给予链脲佐菌素(50mg/kg)复制1型糖尿病模型,将模型大鼠随机分为模型(生理盐水)组、阳性对照(胰岛素注射液2.25 u/kg)组和微乳剂低、中、高剂量组(胰岛素自微乳剂4.5、9、18 u/kg);另取正常大鼠10只为假手术(生理盐水)组。均麻醉手术,除阳性对照组大鼠ip给药外,其余大鼠肠道给药,于给药前和给药后15~600 min检测各组大鼠血糖水平。另分别对正常对照组、模型未给糖组、模型给糖组和微乳剂(胰岛素自微乳剂9 u/kg)组大鼠进行糖耐量实验(除模型未给糖组大鼠不给糖外其余大鼠均给糖)。结果:与假手术组比较,模型组大鼠给药后0~240 min内的血糖值均增加,差异具有统计学意义(P<0.05)。与模型组比较,阳性对照组大鼠给药15 min后有降血糖趋势,给药后30~480 min内的血糖值均减小,其中降血糖峰值为36%、峰值时间为30 min;微乳剂低、中、高剂量组大鼠给药30 min后有降血糖趋势,给药后45~360 min内的血糖值均减小,其中降血糖峰值为18%~21%、峰值时间为90~120min,以上差异具有统计学意义(P<0.01或P<0.05)。糖耐量实验中除模型未给糖组外其余各组大鼠均有血糖吸收峰,且微乳剂组大鼠血糖达峰值后迅速降低。结论:胰岛素自微乳剂能明显降低1型糖尿病模型大鼠的血糖。更多还原

【Abstract】 OBJECTIVE:To study the hypoglycemic effects of Insulin self-microemulsion for parenteral administration on model rats with type 1 diabetes in vivo.METHODS:Rats were treated with streptozotocin(50 mg/kg)to reproduce model with type 1 diabetes,ip.The model rats were randomly divided into model group(normal saline),positive control group(Insulin injection2.25 u/kg)and self-microemulsion low,medium and high dose groups(Insulin self-microemulsion 4.5,9 and 18 u/kg);and 10 normal rats were involved in sham-operation group(normal saline).Anesthesia and operation were conducted for all rats.Positive control group was administrated,ip;other rats were parenterally administrated.The blood glucose levels in groups were detected before and after 15-600 min administration.Glucose tolerance test was conducted for the rats in normal control group,model group without glucose,model group with glucose and microemulsion group(Insulin self-microemulsion 9 u/kg).All group were given glucose except model group without glucose.RESULTS:Compared with sham-operation group,the blood glucose levels in model group within 0-240 min were increased,with significant difference(P<0.05).Compared with model group,there was hypoglycemic trend in positive control group after 15 min,the blood glucose levels within 30-480 min were decreased,the hypoglycemic peak was 36%,and the peak time was 30 min;there was also hypoglycemic trend in microemulsion low,medium and high dose groups after 30 min,the blood glucose levels within 45-360 min were decreased,hypoglycemic peak was 18%-21%,and the peak time was 90-120 min,with significant differences(P<0.01 or P<0.05).All rats had glucose absorption peak except for model group without glucose in glucose tolerance test,and glucose of rats in microemulsion group reached its peak and then quickly decreased.CONCLUSIONS:Insulin self-microemulsion can obviously reduce the blood glucose of model rats with type 1 diabetes.更多还原