【摘要】 目的:研究姜黄素对急性心肌梗死模型大鼠的保护作用并探讨其机制。方法:结扎冠状动脉前降支以复制大鼠急性心肌梗死模型。100只SD大鼠分为假手术(ig,等容生理盐水)组、模型(ig,等容生理盐水)组、溶剂对照[ip,6%乙醇-聚乙二醇400溶液,2.5 ml/kg]组、培哚普利(ig,3 mg/kg)组与姜黄素高、中、低剂量(ip,200、100、50 mg/kg)组,复制模型24 h后开始给药,每天1次,连续4周。测定大鼠血液动力学指标[左室舒张末压(LVEDP)、左室收缩末压(LVSP)、左室内压最大上升/下降速率(±dp/dtmax)];计算左、右心室质量指数(LVMI、RVMI);计算心肌梗死面积(IS),测定心肌胶原面积并计算心肌胶原容积分数(CVF);采用免疫组化法测定大鼠心肌基质金属蛋白酶(MMP)-2的表达。结果:与假手术组比较,模型组大鼠LVSP、±dp/dtmax降低、LVEDP、LVMI、RVMI升高,IS增加,CVF升高,MMP-2表达增强,差异有统计学意义(P<0.01)。与模型组比较,姜黄素高、中、低剂量组大鼠LVSP、±dp/dtmax升高,LVMI、RVMI降低,CVF降低,MMP-2表达减弱;姜黄素高、中剂量组大鼠LVEDP降低,差异有统计学意义(P<0.01或P<0.05)。结论:姜黄素对急性心肌梗死模型大鼠具有一定保护作用,其机制可能与减轻心肌肥厚、减少胶原沉积、降低MMP-2的表达有关。更多还原

【Abstract】 OBJECTIVE:To investigate the protective effect of curcumin on rats with acute myocardial infarction(AMI),and discuss the protection mechanism. METHODS:Anterior descending coronary arteries were ligated to establish rat models of AMI.100 SD rats were randomly divided into sham-operation group(ig,constant volume normal saline),model group(ig,constant volume normal saline),solvent control group [ip,6% alcohol-polyethylene glycol 400 solution,2.5 ml/kg,perindopril group(ig,3mg/kg)and curcumin high,medium and low dose groups(ip,200,100 and 50 mg/kg). They were given the drug 24 h after the establishment of models,once a day for 4 weeks. Haemodynamic indexes [left ventricular end-diastolic pressure(LVEDP)],left ventricular end-systolic pressure(LVSP)and maximum rate of left ventricular pressure rise/decline(±dp/dtmax)] were determined;left and right ventricular mass indexes(LVMI and RVMI)and myocardial infarction areas(IS)were calculated;the areas of myocardial collagen were determined and the collagen volume fractions(CVF)were calculated;and immunohistochemical method was adopted to detect the expression of matrix metalloproteinase-2(MMP-2)in rats. RESULTS:Compared with sham-operation group,the LVSP and ± dp/dtmaxof rates in model group were decreased;LVEDP,LVMI,RVMI,IS,CVF and expression of MMP-2were increased,with significant difference(P<0.01). Compared with model group,the LVSP and ± dp/dtmaxof rats in curcumin high,medium and low dose groups were increased,and LVMI,RVMI,CVF and expression of MMP-2 were decreased;the LVEDP of rats in curcumin high and medium dose groups were decreased,with significant difference(P<0.01 or P<0.05). CONCLUSIONS:Curcumin has certain protective effect on model rats with AMI. The mechanism may be related to the reduction of myocardial hypertrophy,collagen deposition and the expression of MMP-2.更多还原