【摘要】 目的:建立蒲药灌肠液的质量控制方法。方法:采用薄层色谱法对乳香、黄芪甲苷和延胡索乙素进行定性鉴别,采用高效液相色谱法同时测定香蒲新苷和异鼠李素-3-O-新橙皮苷的含量。结果:薄层色谱均检出乳香、黄芪甲苷和延胡索乙素,斑点清晰,专属性强;含量测定中香蒲新苷和异鼠李素-3-O-新橙皮苷的线性范围分别为0.216 0~3.240μg(r=0.999 9,n=6)和0.208 8~3.132μg(r=0.999 8,n=6),加样回收率分别为98.6%(RSD=1.3%,n=6)和98.4%(RSD=1.4%,n=6)。结论:该方法简便可行,结果准确可靠,可用于蒲药灌肠液的质量控制。更多还原

【Abstract】 OBJECTIVE:To establish a method for the quality control of Puyao rectal solution. METHODS:Thin layer chromatography(TLC)method was used to identify frankincense,astragaloside A and tetrahydropalmatine;HPLC was used to determinate the content of typhaneoside and isorhamnetin-3-O-neohesperidoside. RESULTS:Frankincense,astragaloside A and tetrahydropalmatine were detected by TLC with clear spot and strong specificity. The linear ranges of typhaneoside and isorhamnetin-3-O-neohesperidoside were respectively 0.216 0-3.240 μg(r=0.999 9,n=6)and 0.208 8-3.132 μg(r=0.999 8,n=6),the average recoveries were respectively 98.6%(RSD=1.3%,n=6)and 98.4%(RSD=1.4%,n=6). CONCLUSIONS:The method is simple,accurate,and can be used for the quality control of Puyao rectal solution.更多还原