【摘要】 目的:研究皮鳞癌1号方对人皮肤鳞状癌A431细胞生长的抑制作用。方法:以0、5、10、20、40、80μg(生药)/ml皮鳞癌1号方分别作用A431细胞24、48、72 h后,测定光密度并计算细胞增殖抑制率以评价其抑制作用;在显微镜下观察细胞形态学变化;光化学法测定乳酸脱氢酶(LDH)的活性;免疫细胞化学法测定B淋巴细胞瘤2(Bcl-2)蛋白、Bcl-2相关X蛋白(Bax)的表达。结果:5、10、20、40、80μg(生药)/ml皮鳞癌1号方对A431细胞均有抑制作用(P<0.01);5、40μg(生药)/ml皮鳞癌1号方可使A431细胞增殖变缓,细胞出现皱缩,与周围细胞黏附力减弱;5、10、20μg(生药)/ml可明显增强A431细胞LDH活性、Bax表达,减弱Bcl-2表达,降低Bcl-2/Bax的比值(P<0.01)。结论:皮鳞癌1号方对A431细胞的增殖具有一定的抑制作用;可能与促进Bax表达、抑制Bcl-2表达、降低Bcl-2/Bax的比值有关。更多还原

【Abstract】 OBJECTIVE:To study the inhibitory effects of Skin scaly formulation No.1 on the growth of the human skin squamous carcinoma A431 cell.METHODS:A431 cells were treated with different concentrations of Skin scaly formulation No.1 [0,5,10,20,40 and 80 μg(medicinal materials)/ml] for 24,48 and 72 h;the morphological changes of cells were observed under microscope.The activity of lactate dehydrogenase(LDH)was measured by spectroscopy;the expressions of Bcl-2 and Bax were determined by immunohistochemistry assay.RESULTS:Skin scaly formulation No.1 [5,10,20,40 and 80 μg(medicinal materials)/ml] could inhibit A431 cell.Skin scaly formulation No.1 [5 and 40 μg(medicinal materials)/ml] could slow down the proliferation of A431 cell,induced cell shrinkage and weakened cell adhesion.Skin scaly formulation No.1 [5,10 and 20 μg(medicinal materials)/ml] could increase the activity of LDH and Bax expression,and decreased the expression of Bcl-2 and Bcl-2/Bax(P<0.01).CONCLUSIONS:Skin scaly formulation No.1 can inhibit the proliferation of A431 cells,which may be associated with that it improves Bax expression,inhibits Bcl-2 expression and decreases Bcl-2/Bax.更多还原