【摘要】 目的:建立三黄糖敏汤中多糖含量的测定方法。方法:采用蒽酮-硫酸比色法。以无水葡萄糖为对照品,在625 nm处测定吸光度。蒽酮-硫酸试剂用量为4.0 ml,沸水浴加热时间为5 min。结果:葡萄糖的质量浓度在5.0~13.4μg/ml范围内与吸光度呈良好的线性关系(r=0.998 5);精密度试验的RSD<2%,重复性、稳定性试验的RSD<3%;平均加样回收率为93.8%,RSD为2.2%(n=6)。三黄糖敏汤中多糖的平均质量浓度为8.6 mg/ml。结论:该方法简单、准确、重复性好,可用于三黄糖敏汤中多糖含量的测定。更多还原

【Abstract】 OBJECTIVE:To establish a method for the content determination of polysaccharides in Sanhuang tangmin decoction. METHODS:The anthrone-sulfuric acid colorimetric method was used. Anhydrous glucose was employed as the standard reference and the absorbance was measured at the wavelength of 625 nm. The volume of the anthrone-sulfuric acid solution was 4.0 ml and heating time was 5 min. RESULTS:The glucose contents showed the fine linearity relation with the absorbance in the range of5.0-13.4 μg/ml(r=0.998 5)with an average recovery of 93.8%(RSD=2.2%,n=6). RSD of precision test was lower than 2%,and RSDs of reproducibility and stability tests were all lower than 3%. The average concentration of polysacharides in Sanhuang tangmin decoction was 8.6 mg/ml. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the content determination of polysaccharide in Sanhuang tangmin decoction.更多还原