【摘要】 本实验旨在研究A-FABP基因在脂肪型和瘦肉型北京鸭不同组织的m RNA表达情况及在脂肪组织不同时期的m RNA表达差异。分别采集6周龄心脏、肝脏、脾脏、肾脏、肌胃、十二指肠、胸肌、腿肌组织样品,另外分别采集2、4、6、8周龄脂肪型和瘦肉型北京鸭的皮脂、腹脂样品,用荧光定量PCR方法检测该基因的m RNA表达情况。结果表明:鸭A-FABP基因主要在脂肪组织中表达,皮脂和腹脂中的表达量远远高于其他组织(P<0.05),6周龄瘦肉型北京鸭A-FABP基因在不同组织中表达量顺序为皮脂>腹脂>肌胃>十二指肠>心脏>腿肌>胸肌>脾>肝>肾,而脂肪型北京鸭顺序为皮脂、腹脂>肌胃>十二指肠>心脏、腿肌>胸肌、脾>肝>肾;且脂肪型北京鸭A-FABP基因在皮脂和腹脂中表达量显著低于瘦肉型北京鸭(P<0.05);A-FABP基因在皮脂和腹脂中的表达量随着周龄的增加呈现增加的趋势。更多还原

【Abstract】 An experiment was conducted to evaluate A-FABP gene express characteristic in various tissues of both fat strain and lean strain ducks and the gene expression difference of adipose tissues in different period. Heart, liver,spleen, kidney, muscular stomach, duodenum, pectoral muscle, thigh muscle, sebum, abdominal fat tissues on 6-week-old samples of two stain ducks, as well as sebum and abdominal fat tissues on 2, 4, 6, 8-week-old of two types of ducks, were collected respectively to detect A-FABP gene expression patterns by q-PCR. The results showed that A-FABP gene express highly in adipose tissue of 6-week-old ducks, and duck A-FABP gene express much higher in sebum and abdominal fat tissues than other tissues(P <0.05); 6-week-old lean strain ducks A-FABP gene express quantity in various tissues as follows: sebum >abdominal fat >muscular stomach >duodenum, heart, thigh muscle >pectoral muscle >spleen, liver >kidney, while fat strain ducks as follows: sebum, abdominal fat >muscular stomach >duodenum>heart, thigh muscle>pectoral muscle, spleen>liver>kidney; 6-week-old lean strain ducks had a greater AFABP gene expression in sebum and abdominal fat tissues than those of fat strain ducks( P <0.05); two types of ducks A-FABP gene expression in both sebum and abdominal fat tissues increase with age increase.更多还原