【摘要】 目的探讨细胞外调节蛋白激酶(ERK)1/2信号转导通路在βTC-6细胞葡萄糖刺激胰岛素分泌(GSIS)反应中的作用。方法采用不同浓度葡萄糖刺激βTC-6细胞,放射免疫法检测细胞上清液中胰岛素浓度,Western blot检测细胞裂解物ERK1/2磷酸化水平。采用MEK抑制剂PD98059处理βTC-6细胞,放射免疫法检测葡萄糖刺激后上清液中胰岛素浓度,Western blot检测葡萄糖刺激后ERK1/2磷酸化水平。结果βTC-6细胞在1.38mmol/L葡萄糖刺激时,胰岛素分泌和ERK1/2磷酸化水平达高峰。PD98059可抑制葡萄糖刺激下ERK1/2磷酸化及胰岛素分泌,作用与剂量呈正相关。结论ERK1/2信号转导通路可能在βTC-6细胞GSIS中发挥作用。更多还原

【Abstract】 Objective To investigate the effect of extracellular signal-regulated kinase 1/2(ERK1/2)signal transduction pathway on glucose stimulated-insulin secretion in βTC-6 cell line. Methods βTC-6 cells were stimulated with different concentration of glucose.Supernatants insulin was assayed by an insulin RIA kit,and cell lysate was used for protein assay by Western blot.βTC-6 cells were pretreated with MEK inhibitor PD98059 and stimulated with 1.38 mmol/L glucose.And then,supernatants insulin was assayed by Western blot. Results The insulin level and ERK1/2 phosphorylation reach a peak in response to 1.38mmol/L glucose stimulation in βTC-6 cells.MEK inhibitor PD98059 can inhibit insulin secretion and ERK phosphorylation which was induced by glucose stimulation in a dose-dependent manner. Conclusion ERK1/2 signal transduction pathway activation may have an important effect on glucosestimulated insulin secretion in βTC-6 cells.更多还原