【摘要】 为建立简便快速、特异检测沙门菌的方法,根据沙门菌invA基因序列,分别设计特异的捕获探针和检测探针,通过探针与目标rRNA结合,优化反应条件,建立了检测沙门菌的夹心DNA杂交方法。该方法在1.5h内即可完成全过程检测,加入TMB显色液可直接肉眼观察结果或采用酶标仪测定D450nm值判定结果。该方法对其他相关病原菌均无特异性反应,最低检测限为1.2CFU/mL,具有良好的精确度。批内变异系数在16.53%~17.97%之间,批间变异系数在16.33%~22.10%之间。本方法的建立为沙门菌的大批量、快速检测和防控提供了新的技术手段。更多还原

【Abstract】 A novel DNA hybridization technology was established for the rapid and specific detection of Salmonella using a capture probe and a detector probe,respectively,which were targeted to the invAgene.The process was completed in less than 1.5hours and the result was determined by visual inspection after adding soluble TMB substrate solution or was tested the D450 nmvalue by multimode reader.No false-positive results were observed for the other related bacteria used to evaluate assay specificity.The detection limit of the assay was up to 1.2CFU/mL.The intra-assay coefficient of variation was between 16.53%and17.97%.The inter-assay coefficient of variation was between 16.33% and 22.10%.The assay provides a new technical way for rapid detecting and preventing infection caused by Salmonella.更多还原