【摘要】 为探明腺胃型传染性支气管炎病毒(IBV)对鸡的致病性,采用滴鼻、点眼和滴口方法,用QXIBV、DYIBV、FCIBV分离毒株对未免疫鸡和SPF鸡进行了攻毒致病回归试验。结果显示,未免疫的10日龄鸡攻毒后致死鸡的剖检变化与20日龄未免疫的被攻毒鸡相似,均有腺胃肿大,腺胃黏膜出血、溃疡,肾肿大、尿酸盐沉积等病变;20日龄(高日龄)攻毒鸡较低日龄(10日龄)攻毒鸡死亡率偏高。被攻毒的SPF鸡均出现精神沉郁、羽毛蓬乱、腹泻等与自然发病鸡相同的临床症状和病理变化(腺胃肿大,黏膜出血、溃疡,肾肿大、尿酸盐沉积等病变);被攻毒的未免疫鸡、SPF鸡与自然发病鸡死亡率相似。试验结果表明,用这3株腺胃型IBV分离株均能人工复制出与自然发病鸡基本一致的临床症状和病理变化。更多还原

【Abstract】 In order to understand the pathogenicity of proventriculus type infectious bronchitis virus(IBV),3IBV strains(QXIBV,DYIBV and FCIBV)were used to challenge the unimmunized chickens and SPF chickens via nose,eye and mouth dripping in regression tests.In result,the post-mortem changes of chickens died from virus challenge in 10-day-old unimmunized were similar to those in 20-day-old unimmunized chickens.Both showed glandular stomach swelling,proventriculus mucosa hemorrhage and ulcer,kidney swelling and uratosis.The mortality of 20-day-old challenged chickens was somewhat higher than that of the 10-day-old challenged chickens.All the challenged SPF chickens showed clinical signs of depression,puffed feather and diarrhea,pathological changes of glandular stomach swelling,proventriculus mucosa hemorrhage and ulcer,and kidney swelling and uratosis which were similar to those in the naturally-infected birds.The result showed that clinical signs and pathological changes similar to those in the naturallyinfected birds could be reproduced using the Shandong IBV isolates.更多还原