【摘要】 为了提高野生白刺(Nitraria sibirica)组培过程中的增殖系数,以野生白刺的无菌苗幼嫩茎段为外植体,利用响应面法对白刺茎段增殖诱导试验进行优化。在添加不同激素浓度NAA、IBA、IAA与6-BA组合的单因素增殖诱导试验基础上,根据CCD中心组合试验设计原理,采用2因素5水平的响应面法,对挑选出的6-BA和IBA两个因素浓度进行优化,得出增殖诱导最佳培养基为MS+6-BA(0.22mg·L-1)+IBA(0.55mg·L-1)。在此条件下,实际增殖系数可达到3.90。优化后的培养基使得增殖系数从之前单因素实验所得最高增殖系数3.63提高到了3.90。为野生白刺组培方面稳定增殖以及后续在组培环境下与锁阳接种研究提供了保障。更多还原

【Abstract】 In order to improve the proliferation index of wild Nitraria sibirica during tissue culture,we selected the young stem segments on aseptic seedling of wild nitraria as the explant to optimize the proliferation induced with the response surface method.On the basis of one-factor proliferation test which were added different concentrations of NAA,IBA,IAA and 6-BA,combinated in medium,and according to the central composite experimental design principles,the response surface method with 2 factors and 5 levels was adopted to optimize the concentrations of 6-BA and IBA which were selected through previous test.The results showed that the best proliferation medium was MS+6-BA(0.22 mg·L-1)+IBA(0.55 mg·L-1).In this medium,the actual proliferation coeffcient was up to 3.90.The optimized medium increased proliferation coeffcient 3.63 which was the best result of one-factor tests to 3.90.This provided guarantee for the stable proliferation in tissue culture of wild nitraria and the follow-up study on combinating with Cynomorium in plant tissue culture environment.更多还原