【摘要】 以珍稀泌盐植物长叶红砂(Reaumuria trigyna)成熟种子和不同发育阶段幼苗的不同部位为外植体,对其愈伤组织诱导和增殖进行了系统研究。结果表明:在添加3.0mg·L-12,4-D、1.0mg·L-1 NAA、0.5mg·L-1 6-BA、30g·L-1蔗糖和6.0g·L-1琼脂,pH为5.8的MSO培养基中,出愈率达70%以上,生长量高于其他培养基,是该植物愈伤组织诱导的最佳培养体系;7d下胚轴和25d的幼苗较适合愈伤组织诱导,可形成淡黄色疏松愈伤组织,且褐化程度相对较低;适宜的继代周期为30~40d,随着传代次数增加,愈伤组织颜色变淡,生长速度加快;NaCl浓度为50mmol·L-1和100mmol·L-1时促进愈伤组织生长,盐浓度过高则导致其生长受抑,甚至死亡。更多还原

【Abstract】 The aim of this study was to evaluate callus induction and proliferation in the endangered recretohalophyte Reaumuria trigyna.Mature seeds and seedlings at different development stages were used as the ex-plant materials.The best medium for callus formation and proliferation was improved MSO(pH5.8)supplemented with 3.0 mg·L-1 2,4-dichlorophenoxyacetic acid,1.0 mg·L-1 naphthaleneacetic acid,0.5 mg·L-1 6-benzylaminopurine,30 g·L-1 sucrose,0.5 g·L-1 casein hydrolysate,and 6.0 g·L-1 agar.On this medium,the callus induction rate was greater than 70%,and the growth rate was higher than those of ex-plants on other media.The optimal ex-plants for callus induction were hypocotyls from7-day-old and 25-day-old seedlings.The calli that formed from these ex-plants were loose,light yellow,and showed a lower browning rate,compared with calli that grew from other ex-plants.The optimum duration of the subculture cycle was 30-40 days.With longer passage times,the callus grew faster and was a lighter color.When calli were cultured on media with different NaCl concentrations,their growth was accelerated on media containing 50 and 100 mmol·L-1 NaCl,but higher concentrations of NaCl inhibited callus growth or resulted in callus death.更多还原