【摘要】 以69份类型明确的亚洲栽培稻品种为材料,选用120对SSR引物,通过评估遗传多样性和群体结构,研究分析其遗传变异对SSR引物数的要求。结果表明:1)120对引物在69份试验材料中共检测到1256个等位变异,每个位点的等位基因数差异较大,变化范围为2~27,平均为10.5;平均Nei基因多样性指数变化范围为0.4058~0.9452,平均为0.7616;2)分析亚洲栽培稻遗传多样性时,至少需要72对SSR引物;3)分析亚洲栽培稻群体结构时所需最少引物数可降低至60对。更多还原

【Abstract】 A total of 120 SSR markers were used to determine the minimum of SSR markers for analyzing genetic variation of 69 Oryza sativa L.accessions,which have been clearly classified,based on the genetic diversity and population structure.The results showed that:1)a total of 1,256 alleles were detected with 120 SSR markers.The number of alleles per locus widely ranged from 2to 27 with an average of 10.5.Nei′s genetic diversity index per locus varied from 0.413 to 0.951 with a mean of 0.760;2)the minimum of SSR markers for analyzing genetic diversity of rice was 72;3)the number of SSR markers for analyzing population structure of rice was at least 60.更多还原