【摘要】 目的探讨蛋白激酶C(PKC)α和PKCβ特异性抑制剂Go6976、PKCβ特异性抑制剂LY333531和Rho激酶特异性抑制剂Y-27632对宫缩乏力性产后出血产妇子宫平滑肌收缩的抑制作用。方法 2010年11月至2011年12月于福建省妇幼保健院选择剖宫产分娩的初产妇60例,分宫缩乏力性产后出血组(病例组,n=30)和宫缩良好无产后出血组(对照组,n=30)。采用肌条等长张力测定方法检测两组产妇子宫下段平滑肌分别在Go6976、LY333531和Y-27632作用下自发性收缩功能变化及病例组在LY333531、Y-27632作用下梯度浓度缩宫素诱导的收缩潜能变化。结果 Go6976、LY333531和Y-27632均显著抑制两组产妇子宫平滑肌收缩幅度及活动力(均P<0.05);对病例组抑制作用均小于对照组(均P<0.05),而对两组的收缩频率无影响(P>0.05)。LY333531和Y-27632均可显著降低病例组收缩潜能(P<0.05)。结论 PKCα、PKCβ和Rho激酶影响子宫平滑肌收缩,可能参与宫缩乏力性产后出血的发生。更多还原

【Abstract】 Objective To investigate the effects of Go6976(PKCα/β inhibitor),LY333531(PKCβ inhibitor)and Y-27632(Rho kinase inhibitor)on human uterine contractility in atonic postpartum hemorrhage(PPH). Methods Totally30 women with atonic PPH as study group and 30 normal pregnant women as control group were included in this study.The myometrial strips from atonic PPH women were adopted to investigate their inhibitory effects by adding Go6976,LY333531 and Y-27632 with isometric tension recording;meanwhile,the effects of LY333531 and Y-27632 on contraction induced by oxytocin were observed following atonic PPH,respectively.Results In study group,the inhibitory effects of Go6976,LY333531 and Y-27632 on amplitude and activity of spontaneous oscillatory contractility were significantly lower than in control group(P<0.05). There was no significance in frequency between two groups(P>0.05).LY333531 and Y-27632 further decreased myometrial strips contraction potentiality induced by oxytocin significantly after PPH(P<0.05). Conclusions PKCα,PKCβ and Rho kinase may be involved in the regulation of myometrial strips contraction,and play an important role in the pathogenesis of atonic PPH.更多还原