【摘要】 目的制作肝脏特异性高表达人胆固醇酯转移蛋白(CETP)转基因家兔并对其生物学特性分析进行分析。方法利用显微注射方法制作CETP转基因家兔,利用Western blot、real-time PCR和CETP活性鉴定试剂盒鉴定模型家兔中人CETP转基因的表达和活性。结果 PCR检验结果显示成功获得CETP转基因家兔,转基因主要在肝脏特异性表达,其血浆CETP活性相对于非转基因家兔明显升高。结论成功建立了高表达人CETP的转基因家兔,为研究CETP功能和其参与心血管疾病的机制提供了良好模型。更多还原

【Abstract】 Objective The aim of this study was to generate human cholesteryl ester transfer protein( CETP)transgenic rabbits and analyze their biological properties. Methods We generated human CETP transgenic rabbits by DNA microinjection,and detected the expression of human CETP by real-time PCR and Western blot assay. The activity of CETP was measured using an activity assay kit. Results Human CETP transgenic rabbits were successfully generated by DNA microinjection. Compared with wide type rabbits,the expression of human CETP was dramatically increased in the liver of the human CETP transgenic rabbits. The plasma CETP activity was also much higher in the liver of human CETP transgenic rabbits than that of control rabbits. Conclusions The model of human CETP transgenic rabbits is successfully established by DNA microinjection. It will provide a useful tool for the studies of CETP biological function and its involvement in the mechanisms of cardiovascular diseases.更多还原