【摘要】 目的通过对脾肾阳虚型溃疡性结肠炎(UC)模型大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ表达水平的检测,探讨它们在UC发生发展过程中的作用。方法采用灌服大黄水煎液+肌肉注射氢化可的松并结合TNBS(2,4,6-三硝基苯磺酸)+乙醇灌肠建立脾肾阳虚型UC动物模型。将60只大鼠随机分为空白组、脾肾阳虚型UC模型7、14d及21d组,采用酶联免疫法检测各组大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ的含量。结果与空白组比较,脾肾阳虚型UC模型组大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ含量明显升高(P<0.05);尤以模型21d组最为明显。结论促炎性细胞因子IL-1、IL-6、TNF-α及IFN-γ在脾肾阳虚型UC发病过程中起重要作用。更多还原

【Abstract】 Objective To detect the levels of IL-1,IL-6,TNF-α and IFN-γ in serum and colon tissue of rat models of ulcerative colitis with spleen and kidney Yang deficiency,and to explore their roles in the pathogenesis of ulcerative colitis( UC). Methods The rat model of ulcerative colitis with Yang deficiency of spleen and kidney was induced by perfusion of rhubarb decoction plus intramuscular injection of hydrocortisone and combined with TNBS( 2,4,6-trinitrobenzenesulfonic acid) and ethanol enema. Sixty SPF wistar rats( body weight 180 ± 10 g,male: female = 1: 1) were randomly divided into blank control group,UC model with spleen kidney Yang deficiency for 7 days,14 days and 21 days groups,respectively. The levels of IL-1,IL-6,TNF-α and IFN-γ in serum and colon tissue were detected by ELISA. Results Compared with the blank group,the levels of IL-1,IL-6,TNF-α and IFN-γ in serum and colon tissue of rat UC model group with spleen kidney Yang deficiency were greatly increased( P < 0. 05),especially evidently increased in the model group at 21 days. Conclusions The pro-inflammatory cytokines IL-1,IL-6,TNF-α and IFN-γ play an important role in the pathogenesis of ulcerative colitis with syndrome of spleen and kidney Yang deficiency.更多还原