In this study,plantlet bulblet of Longya Lily(Lilium brownii var. viridulum Baker)were used as experimental material to optimize protoplasts preparation parameters by orthogonal experiment.Effects of vibrating speed,enzymolysis time,centrifugal speed of harvesting protoplasts,and the sucrose concentration,etc.factors influencing yield of preparing protoplasts were discussed.The results showed that the optimum conditions were combined by 1.0% of cellulase, 1.0% of pectinase enzymolysis subcultured for 40 d i...