【摘要】 研究以鸭15日胚龄肝脏细胞为试验素材,以终浓度为5 ng/m L的三碘甲腺原氨酸(T3)稀释液及等体积的生理盐水对肝细胞干扰36 h,依次将两组细胞设定为试验组和对照组。采用Cell counting kit-8(CCK-8)法和实时荧光定量PCR方法分别检测干扰后培养0、12、24 h时细胞活性及Ⅲ型脱碘酶(D3)mRNA表达。结果表明,在三个检测时间点,试验组肝细胞活性均显著高于对照组(P=0.026,P=0.006,P=0.013),且在干扰后培养24 h时两组细胞活性在组内均显著高于0和12 h(P=0.000,P=0.000);0和12 h时两组细胞中D3 mRNA表达量在组间和组内均没有发生显著变化,24 h时对照组肝细胞中D3 mRNA表达量显著下调且显著低于试验组(P=0.000,P=0.000)。结论:外源性T3能够促进鸭15日胚龄肝脏细胞的生长,D3在肝细胞增殖过程中可能发挥正向调控的作用。更多还原

【Abstract】 In this study,liver cells of duck at 15 th embryo days were cultured,and the same volume of T3(final concentration 5 ng/m L)and physiological saline were added into the cells cultured for 36 h,defined as interference and control groups,respectively. The cell activity and D3 mRNA expression in cells were evaluated by CCK-8 and Real-time fluorescent quantitative PCR(RT-QPCR)at 0,12 and 24 h after T3 interference,respectively. The results showed that the activity of the treatment cells were significant higher than that of control groups at three times(P=0.026,0.006,0.013),and the activity at 24 h in two groups were all higher than that at 0 and 12 h(P=0.000,P=0.000,). There was no significant difference for all possible combinations of groups at 0 and 12 h. The expression of D3 mRNA in control group at 24 h descended and was significant lower than that of control groups at 0 and 12 h(P<0.01)and the interference group at 24 h(P=0.000). These results indicated that the exogenous T3 could promote the growth of duck embryo liver cells,and D3 might play a positive role in the proliferation of liver cells.更多还原