【摘要】 目的了解吉林省2012~2013年诺如病毒(Norovirus,NV)流行型别及感染状况。方法收集长春市儿童医院2012~2013年<5岁住院腹泻患儿的粪便标本,采用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)检测粪便标本中的NV,阳性标本进行核苷酸序列测定,构建系统进化树进行分析。结果检测粪便标本共770份,NV阳性139份,总阳性率为18.1%,其中2012年总阳性率为18.6%(72/387),2013年总阳性率为17.5%(67/383),<2岁婴幼儿感染占总标本数的97.1%,全部为基因Ⅱ组(GenogroupⅡ,GⅡ)。PCR产物进行序列测定,取得测序结果的共97份,其中GⅡ.4型53份,占54.6%;GⅡ.3型41份,占42.3%;GⅡ.13型2份,占2.1%;GⅡ.6型1份,占1.0%。2012年检出GⅡ.4 Sydney(悉尼)2012变异株2份,占2012年阳性总数的4.9%;2013年检出26份,占2013年阳性总数的46.4%。结论吉林省存在多种NV基因型的感染,2013年流行优势株为GⅡ.4 Sydney2012变异株。更多还原

【Abstract】 Objective To describe the genotypes and situation of norovirus( NV) infection in Jilin province in 2012 and 2013. Methods Stool samples from children under 5 years of age with diarrhea were collected from Changchun Children’s Hospital. We detected NV using reverse transcription-polymerase chain reactions( RT-PCR). Positive samples were sequenced and the phylogenetic tree was constructed for analysis. Results In total,139 of 357 stool samples were positive for NV ribonucleic acid. The total positive rate was 18. 6% in 2012 and 17. 5% in 2013. Children under 2 years of age accounted for97. 1% of the all infected samples,and all strains belonged to genogroup II( GⅡ). The PCR products were sequenced,and for 97 samples we obtained sequencing results. Fifty-three strains( 54. 6%) belonged to genotype GⅡ. 4; 41 strains( 42. 3%) belonged to genotype G Ⅱ. 3; 2 strains( 2. 1%) belonged to genotype GⅡ. 13; and 1 strain( 1. 0%) belonged to genotype GⅡ. 6. Two mutant GⅡ. 4 Sydney2012 strains were detected in 2012,accounting for 4. 9% of positive samples. Twenty-six of the GⅡ. 4 Sydney2012 strains were detected in 2012,accounting for 46. 4% of the total positive samples. Conclusion There were many kinds of NV genotype infections in Jilin province,and GⅡ. 4 Sydney2012 strain became the predominant one in 2013.更多还原