【摘要】 目的探讨宫颈癌组织中的B淋巴细胞瘤(Bcl)-2蛋白、Survivin mRNA,HPV16/18 DNA的表达及其意义。方法收集2009年5月至2013年12月接受治疗的宫颈癌患者和宫颈上皮内瘤变(CIN)179例患者的临床资料,按临床及病理分为宫颈癌组(79例)及CIN组(共69例,其中CINⅠ级14例,Ⅱ级25例,Ⅲ级30例)。另取包括宫颈良性病变的正常宫颈组织31个,作为对照组。比较3组宫颈组织中的HPV16/18 DNA及Bcl-2、Survivin mRNA表达情况,并探讨其与宫颈组织相关病理特征之间的关系。结果 3种宫颈组织中的Survivin mRNA、Bcl-2、HPV16/18 DNA的阳性表达情况组间比较均有统计学差异(P均<0.01),且均为正常宫颈组织阳性表达最低,宫颈癌组织表达最高。Survivin mRNA、Bcl-2以及HPV16/18 DNA阳性表达率与CIN分级有明显相关性(Ⅰ+Ⅱ级vsⅢ级,P均<0.01),CIN分级越高,相应的阳性表达率越高。Bcl-2阳性表达率与宫颈癌的组织类型、分期、肿瘤生长类型无明显相关性(P均>0.05),而与宫颈癌组织的淋巴结转移情况、分化程度有关(P均<0.01);Survivin mRNA阳性表达率与宫颈癌的组织类型、肿瘤生长类型无明显相关性(P均>0.05),而与宫颈癌组织的临床分期(P<0.05)、分化程度(P<0.01)以及淋巴结转移情况(P<0.01)有明显相关性;HPV16/18 DNA的阳性表达率与宫颈癌的组织类型、肿瘤生长类型无明显相关性(P均>0.05),而与组织的临床分期(P<0.05)、分化程度(P<0.01)以及淋巴结转移情况(P<0.01)有明显相关性。结论 HPV16/18 DNA,Bcl-2、Survivin mRNA在宫颈癌组织中的表达均明显升高,可能与宫颈癌的发生、发展有着密切的联系。更多还原

【Abstract】 Objective To investigate the expressions of HPV16 /18 DNA,Bcl-2 and Survivin mRNA in the cervical cancer tissues and the related significance. Methods The clinical data of 179 patients with cervical cancer / cervical intraepithelial neoplasia( CIN) received treatment from May 2009 to December 2013 were collected,and based on the clinical and pathological data,the patients were divided into cervical cancer group( n = 79) and CIN group( n = 69) including 14 cases of CIN stage I,25 cases of CIN stage Ⅱ and 30 cases of CIN stage Ⅲ. Thirty-one specimens of normal cervical tissues including cervical benign lesions were selected as control group. The expressions of HPV16 /18 DNA,Bcl-2 and Survivin mRNA in different cervical tissues were compared,and their associations with related pathological features of cervical tissues were studied. Results There were all statistical differences in the positive expression rates of HPV16 /18 DNA,Bcl-2 and Survivin mRNA on three kinds of cervical tissues( all P < 0. 01),and the lowest and the highest positive expression rates were all normal cervical tissues and cervical cancer tissues,respectively. There were all statistical differences in the positive expression rates of HPV16 /18 DNA,Bcl-2 and Survivin mRNA on the tissues of different CIN staging( stagesⅠplusⅡ vs stage Ⅲ,all P < 0. 01),and the higher the stage,the higher the corresponding positive expression rate. There was no significant relationships between the positive expression rate of Bcl-2 and the type,staging of cervical cancer tissue,the growth type of tumor( all P > 0. 05),but there were significant relationships between the positive expression rate of Bcl-2 and lymph node metastasis,differentiation degree of cervical cancer tissues( all P < 0. 01). There was no significant relationships between the positive expression rate of Survivin mRNA and tissue type of cervical cancer tissue,the growth type of tumor( all P > 0. 05),but there were significant relationships between the positive expression rate of Survivin mRNA and clinical staging( P < 0. 05),differentiation degree( P < 0. 01),lymph node metastasis of cervical cancer tissues( P <0. 01). There were no significant relationships between the positive expression rate of HPV16 /18 DNA and the type of cervical cancer tissue,the growth type of tumor( all P > 0. 05),but there were significant relationships between the positive expression rate of HPV16 /18 DNA and clinical staging( P < 0. 05),differentiation degree( P < 0. 01),lymph node metastasis( P < 0. 01) of cervical cancer tissues. Conclusion All the expressions of Bcl-2,Survivin mRNA and HPV 16 /18 DNA in cervical cancer tissues increase obviously,and this might closely be correlated with the occurrence and development of cervical cancer.更多还原