【摘要】 目的研究宫颈癌细胞整体甲基化水平与放疗敏感性的关系。方法用甲基转移酶抑制剂DAC处理宫颈癌He La细胞系,然后给予不同剂量放疗照射,用克隆形成实验评价He La细胞的放疗敏感性。结果 2.5和5μM浓度DAC处理过的He La细胞放疗敏感性高于未处理的He La细胞(P<0.01)。5μM浓度DAC处理过的He La细胞放疗敏感性高于2.5μM浓度DAC处理过的He La细胞(P<0.01)。结论 DAC对宫颈癌He La细胞系的放疗敏感性具有增强作用。更多还原

【Abstract】 Objective To study the correlation between overall methylation level of cervical cancer cells and radiosensitivity.Methods Methyltransferase inhibitor DAC was used to treat cervical cancer He La cell line,then different doses of radiotherapy irradiation were given,clone formation assay was used to evaluate radiosensitivity of He La cells. Results Radiosensitivities of He La cells treated by2. 5 and 5 μM DAC were statistically significantly higher than that of He La cells without treatment( P < 0. 01). Radiosensitivity of He La cells treated by 5 μM DAC was statistically significantly higher than that of He La cells treated by 2. 5 μM DAC( P < 0. 01).Conclusion DAC can enhance radiosensitivity of cervical cancer He La cell line.更多还原