【摘要】 目的:分析CD31、CD105和PTEN蛋白与宫颈癌临床病理指标的关系,探讨其在宫颈癌发生、发展、转移及预后中的意义及相互关系。方法:采用免疫组化SP法检测65例宫颈癌组织、25例正常宫颈组织中的CD31、CD105标记微血管密度(MVD)情况和PTEN蛋白表达情况。结果:CD31在宫颈癌组织中MVD为(48.33±3.66)高于正常宫颈组织中MVD(8.68±1.26),差异有统计学意义(P<0.05);CD31-MVD值与宫颈癌组织学类型、临床分期有关(P<0.05),而与年龄、淋巴结转移及组织学分化程度无关(P>0.05)。CD105在宫颈癌组织中MVD为(32.56±2.81)高于正常宫颈组织中MVD(1.69±2.14),差异有统计学意义(P<0.05);MVD值与宫颈癌临床分期、有无淋巴结转移有关(P<0.05),而与年龄、组织学分化程度及组织学类型无关(P>0.05)。正常宫颈组织PTEN阳性表达率为100.0%(25/25)高于宫颈癌组织36.92%(24/65),差异有统计学意义(P<0.01)。宫颈癌组织PTEN阳性表达与临床分期、淋巴结转移有关(P<0.05),而与年龄、分化程度、组织类型等无关(P>0.05)。宫颈癌组织中CD31和CD105的表达呈正相关(rs=0.83,P<0.05),CD31和PTEN呈负相关(rs=-0.68,P<0.05),CD105与PTEN呈负相关(rs=-0.76,P<0.05)。结论:联合检测PTEN、CD31与CD105可作为宫颈癌诊断、治疗、转移及评估预后的重要指标。更多还原

【Abstract】 Objective: To analyze the relationship between CD31,CD105,PTEN and clinical pathological parameters of cervical cancer,explore their significance and interaction in occurrence,development,metastasis,and prognosis of cervical cancer. Methods: Immunohistochemical SP method was used to detect CD31 / CD105 marked microvessel density( MVD) and PTEN expression in 65 patients with cervical cancer and 25 patients with normal cervical tissue. Results: MVD of CD31 in cervical cancer was( 48. 33 ± 3. 66),which was statistically significantly higher than that in normal cervical tissue( 8. 68 ± 1. 26)( P < 0. 05). CD31- MVD was correlated with histological types of cervical cancer and TNM stages( P < 0. 05),which was not correlated with age,lymph node metastasis,and histological differentiation degree( P > 0. 05). MVD of CD105 in cervical cancer was( 32. 56 ± 2. 81),which was statistically significantly higher than that in normal cervical tissue( 1. 69 ± 2. 14)( P < 0. 05). CD105- MVD was correlated with clinical staging and lymph node metastasis( P <0. 05),which was not correlated with age,histological differentiation degrees and histological types( P > 0. 05). The positive expression rate of PTEN in normal cervical tissue was 100. 0%( 25 /25),which was statistically significantly higher than that in cervical cancer( 36. 92%,24 /65)( P < 0. 01). PTEN positive expression in cervical cancer was correlated with clinical staging and lymph node metastasis( P < 0. 05),which was not correlated with age,histological differentiation degrees,and histological types( P > 0. 05). In cervical cancer,CD31 expression was positively correlated with CD105 expression( rs= 0. 83,P < 0. 05),CD31 expression was negatively correlated with PTEN expression( rs=- 0. 68,P < 0. 05),CD105 expression was negatively correlated with PTEN expression( rs=- 0. 76,P <0. 05). Conclusion: Joint detection of PTEN,CD31,and CD105 can be used as important parameters for diagnosis,treatment,metastasis,and prognosis assessment of cervical cancer.更多还原