【摘要】 目的 比较传统培养法和PCR-荧光探针法在分枝杆菌的鉴定中有无差异。方法 对1552株分枝杆菌临床分离株同时用传统培养法对硝基苯甲酸(PNB)、噻吩二羧酸肼(TCH)培养基生长试验及PCR-荧光探针法进行鉴定,结果有差别的菌株用16SrRNA基因测序的方法进行确认。结果 两种方法非结核分枝杆菌的符合率为75.4%(46/61),结核分枝杆菌复合群的符合率为99.0%(1491/1506),总体符合率为99.0%(1537/1552)。两种方法检测结果不同的15株样本经16SrRNA基因测序进行鉴定,PCR-探针法与16SrRNA结果一致的有14株,另外l株经传代分离纯化后测序结果为结核分枝杆菌和戈登分枝杆菌的混合菌。非结核分枝杆菌对PNB培养基的敏感度为21.7%(13/60),有0.1%(1/1492)的结核分枝杆菌复合群对PNB表现耐受性。对PNB敏感的13株非结核分枝杆菌中,有76.9%(10/13)为堪萨斯分枝杆菌。结论部分非结核分枝杆菌使用PNB-TCH生长试验进行鉴定时会出现误判,而PCR-荧光探针法鉴定结核分枝杆菌复合群和非结核分枝杆菌的准确性较高。更多还原

【Abstract】 Objective To compare the traditional culture method with PCR-fluorescence probe method for the identification of mycobacteria Methods One thousand five hundred and fifty two mycobacterial strains from clinical isolates were tested by PNB-TCH growth test and PCR-fluorescent probe method,in which the strains with different testing results were confirmed by 16 S rRNA gene sequencing method.Results Between two methods,the coincidence rate of non-tuberculosis mycobacteria(NTM) was 75.4%(46/61),the coincidence rate of Mycobacterium tuberculosis complex(MTBC) was 99.0%(1491/1506),and the overall coincidence rate was 99.0%(1537/1552).15 strains with different testing results were sequenced with 16 S rRNA gene,in which 14 were consistent with the results of PCR-probe method,and 1 was isolated and cultured,then confirmed with mixed bacteria of Mycobacterium tuberculosis and Mycobacterium Gordon.The sensitivity of PNB culture for NTM was 21.7%(13/60),and the PNB-resistant rate of MTBC was 0.1%(1/1492).Of 13 PNB-sensitive NTM stains,10(76.9%)were Mycobacterium kansasii.Conclusion The identification of part NTM isolates was misjudged using the PNB-TCH growth tests,while the PCR-fluorescent probe method for the identification of MTBC and NTM strains had higher accuracy.更多还原