【摘要】 目的探讨婴儿痉挛症患儿免疫功能变化及促肾上腺皮质激素(ACTH)对淋巴细胞亚群的影响。方法 2012年11月至2014年9月济宁医学院附属医院儿科初次确诊的25例婴儿痉挛症患儿作为病例组,21例健康儿童为对照组。病例组给予ACTH治疗4周,治疗前及治疗4周后分别检测其外周血CD3~+T淋巴细胞、CD4~+T淋巴细胞、CD8~+T淋巴细胞和CD4~+/CD8~+值,并与对照组进行比较。结果病例组CD3~+T淋巴细胞为(64.2±7.5)%,低于对照组[(70.2±8.4)%],差异有统计学意义(t=2.53,P<0.05);病例组CD4~+T淋巴细胞为(38.7±4.8)%,低于对照组[(46.8±5.7)%],差异有统计学意义(t=5.15,P<0.05);病例组CD8~+T淋巴细胞为(35.6±5.7)%,对照组为(34.4±4.9)%,差异无统计学意义(t=0.77,P>0.05);病例组CD4~+/CD8+值为1.4±0.3,对照组为1.7±0.5,差异有统计学意义(t=2.41,P<0.05);病例组CD3+T淋巴细胞治疗前为(64.2±7.5)%,治疗后为(64.9±8.6)%,差异无统计学意义(t=0.32,P>0.05);病例组CD4~+T淋巴细胞治疗前为(38.7±4.8)%,治疗后为(39.2±4.6)%,差异无统计学意义(t=0.41,P>0.05);病例组CD8+T淋巴细胞治疗前为(35.6±5.7)%,治疗后为(35.9±5.9)%,差异无统计学意义(t=0.21,P>0.05);病例组CD4~+/CD8~+值治疗前为1.4±0.3,治疗后为1.5±0.5,差异无统计学意义(t=0.80,P>0.05)。结论婴儿痉挛症患儿存在免疫功能紊乱,ACTH对其淋巴细胞亚群无影响,未影响患儿细胞免疫功能。更多还原

【Abstract】 Objective To explore the change of immune function and the effect of Adrenocorticotropic hormone( ACTH) on lymphocyte subsets in children with infantile spasm. Methods Twenty-five cases with newly diagnosed infantile spasm were gathered from November 2012 to September 2014,while another twenty-one healthy cases were chosen as normal control group.ACTH was administered for 4 weeks.Sera was sampled for analyses of the counts of CD3~+,CD4~+ and CD8~+ before and after 4 weeks of treatment,and the results were compared with those of the controls.Results Compared to the controls,the count of CD3~+T cell in patients demonstrated significant decreasement [patients:( 64. 2 ± 7. 5) %,controls:( 70. 2 ± 8. 4) %,t =2.53%,P <0.05].The count of CD4~+T cell in patients demonstrated significant reduction [patients:( 38.7±4.8) %,controls:( 46.8±5.7) %,t = 5.15,P <0.05].The count of CD8~+T cell had no statistically significant differences between patients and controls [patients:( 35. 6 ± 5. 7) %,controls:( 34. 4 ± 4. 9) %,t = 0. 77,P >0.05].The ratio of CD4~+/ CD8~+T cell in patients demonstrated significant decrease [patients: 1. 4 ± 0. 3,controls: 1.7±0.5,t = 2. 41,P < 0. 05]. The count of CD3~+T cell in patients had no statistically significant differences before and after treatment [before treatment:( 64.2±7.5) %,after treatment:( 64.9± 8.6) %,t =0.32,P >0.05]. The count of CD4~+T cell in patients had no statistically significant differences before and after treatment [before treatment:( 38.7 ± 4. 8) %,after treatment:( 39. 2 ± 4. 6) %,t = 0. 41,P > 0. 05]. The count of CD8~+T cell in patients had no statistically significant differences before and after treatment( before treatment:( 35.6±5.7) %,after treatment:( 35.9±5.9) %,t = 0.21,P >0.05).The ratio of CD4~+/ CD8~+T cell in patients had no statistically significant differences before and after treatment( before treatment: 1.4± 0.3,after treatment: 1.5±0.5,t = 0.80,P >0.05).Conclusions Children with infantile spasm have immunological alterations,and ACTH has no effect on lymphocyte subsets. ACTH may not regulate the cellular immune function of children with infantile spasm.更多还原