【摘要】 目的研究芍药总苷(TGP)脂质体的最佳制备工艺及其体外释放情况。方法采用逆向蒸发法制备TGP脂质体,以包封率为评价指标,采用Box-Behnken Design-响应面优化法(BBD-RSM)优化最佳制备工艺参数,并对其体外释放情况进行考察。结果最佳处方为胆固醇与磷脂质量比为1∶2.7,TGP与脂类质量比为1∶29,水化介质用量5.1 m L,药物的平均包封率为73.0%,体外12 h累积释放率为42.18%。结论 TGP脂质体制备工艺合理可行,包封率高,稳定性好,有良好的缓释作用。更多还原

【Abstract】 Objective To optimize the preparation technology for total glucosides from Paeonia lactiflora(TGP) liposome and study on its in vitro release. Methods Liposomes were prepared by reverse-phase evaporation technique. Taking the entrapment efficiency(EE) as indexes, the preparation of liposome was optimized using Box-Behnken design-response surface method(BBD-RSM), and the in vitro release was studied. Results The optimal formulation was cholesterol-lecithin(1∶2.7), TGP-lipid(1∶29), and hydrated medium of 5.1 m L. The average EE were 73.0% and the 12 h in vitro accumulative release rate was 42.18%. Conclusion The optimized process is rational and feasible, and the TGP liposome has the higher EE and good stability with better sustained release in vitro.更多还原