【摘要】 目的我们曾经用淀粉-琼脂混合凝胶电泳证明成人血红蛋白A1与A2发生交叉互作,当时未对胎儿血红蛋白F进行研究。现在,再用这种方法研究血红蛋白F与血红蛋白A2是否也有相互作用。方法由脐带血分离红细胞,再用淀粉-琼脂糖混合凝胶电泳制备纯的血红蛋白F,然后让血红蛋白F穿过成人红细胞溶血液,观察穿过溶血液后血红蛋白A2区带变形情况。结果血红蛋白F穿过成人红细胞溶血液中的血红蛋白A2时,后者变形,与游离血红蛋白F相比,进入溶血液中的血红蛋白F泳速稍慢。结论血红蛋白F与血红蛋白A2也能发生交叉互作。更多还原

【Abstract】 Objective We have demonstrate that adult hemoglobin Hb A1 and Hb A2 can cross interact each other by starch-agarose mixed gel electrophoresis,but the fetal hemoglobin Hb F was not studied at that time.At present,we use this method to study whether the interaction between Hb F and Hb A2 occur.Methods Separating red blood cells from the umbilical cord,then preparation pure hemoglobin F from the starch-agarose electrophoresis gel electrophoresis,and let hemoglobin F dissolve blood through adult red blood cells was observed after dissolving blood hemoglobin A2 through the deformation zone.Results The dissolution of erythrocyte hemoglobin F in the blood through the adult hemoglobin A2,which is deformed as compared with the free hemoglobin F,hemoglobin in the blood solution into the F swimming speed slower.Conclusion The results showed that the cross interaction was occurred as well between Hb F and Hb A2.更多还原