The research established a cDNA-AFLP analysis system which suited Paeonia lactiflora seed by using hybrid seeds of P.‘Fenyunu’ and P.‘Fenzhonglou’ as materials and optimizing the important factors which affect the reaction of cDNA-AFLP. In addition,we made a preliminary analysis on the expression difference of gene under the conditions of before and after breaking epicotyl and hypocotyl dormancy. The results indicated that total RNA was isolated from Paeonia lactiflora seeds using the RNAprep Pure Plant Kit...
【基金】
国家自然科学基金项目(31240028,31470696)
【更新日期】
2015-04-24
【分类号】
S682.12
【正文快照】
基于c DNA的扩增片段长度多态性指纹图谱(c DNA-based amplified fragment lengthpolymorphism fingerprinting,c DNA-AFLP)是Bachem等(1996)在AFLP技术的基础上发明的一种用于研究基因表达差异的RNA指纹分析技术。该技术无需预先知道序列信息,具有假阳性低、重复性好、可靠性?
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