【摘要】 利用混合静电纺丝将葡萄糖淀粉酶(GA)固定于聚丙烯酸(PAA)/聚乙烯醇(PVA)纳米纤维膜上,并通过鉴定固定化GA的酶学特征检验PAA/PVA可否成为一种优良的酶固定化载体。对其理化性质和酶学特征进行鉴定,经红外光谱(FT-IR)和扫描电子显微镜(SEM)表征发现,GA可成功包埋于PAA/PVA纳米纤维膜内部;对包裹固定的GA进行酶学性质鉴定,发现固定化GA的最适反应温度为68℃,比游离GA提高了9℃;固定化GA的适用p H值范围明显变宽;热稳定性和存贮稳定性显著增强且可以重复使用。PAA/PVA纳米纤维膜是一种优良的酶固定化载体,可以通过混合静电纺丝包埋法简便地将蛋白质分子固定于其内部,具有一定的应用前景。更多还原

【Abstract】 Glucoamylase( GA) was entrapped and immobilized in the poly( acrylic acid)( PAA)/polyvinyl alcohol( PVA) nanofibers membrane by the mixed liquid electrospinning entrapping method. The enzyme immobilization potential of PAA/PVA was investigated through appraised enzymatic characterization of immobilized GA. Its physicochemical properties and enzymatic characterizations were identified. Fourier transform infrared spectrum( FT-IR) and scanning electron microscope( SEM) photographs indicate that GA can be successfully entrapped in the interior of the electrospinning PAA/PVA composite nanofiber membrane.The optimum reaction temperature of immobilized GA is 68 ℃,9 degrees higher than that of free GA,and the applicable p H range of immobilized GA is wider than that of free GA significantly. In addition,immobilized GA also has good thermal stability,storage stability and reusability. PAA/PVA nanofiber membrane is a good carrier for enzyme immobilization with potential application. The protein molecules can be easily fixed into the inside of nanofiber membrane via mixed liquid electrospinning entrapping method.更多还原