【摘要】 以四川藏区和攀西松茸代表产区菌塘土壤为研究对象,测定菌塘土壤细菌、放线菌和真菌的菌落数量,土壤微生物的生物量碳和氮,以及土壤酸性磷酸酶、脲酶和过氧化氢酶活性,采用多元回归树分析生态环境因子对微生物的影响.结果表明:当海拔高于3 592 m时,在一定范围内海拔越高(3 841-3 860 m),土壤酶活性越高;当海拔低于3 592 m时,枯枝落叶层厚度越厚(2.25-8.45 cm)、海拔越低(3058-3226 m),土壤酶活性越高.当坡度大于或等于42.5°时,一定范围内枯枝落叶层厚度越高(1.05-8.45 cm)、海拔越低(3058-3225 m),松茸菌塘微生物生物量及菌落数量越高;而当坡度小于42.5°时,在一定范围内海拔越低(3058-3460 m)、枯枝落叶厚度越薄(0.28-3.2 cm)越有利于松茸菌塘土壤微生物生物量和菌落数量积累.总之,海拔、坡度及枯枝落叶厚度是影响松茸菌塘土壤微生物活性和生物量的3个关键因素.更多还原

【Abstract】 This research investigated soil samples of Tricholoma matsutake shiroes in Tibetan region and Panxi region of Sichuan Province to understand the effect of microbial activities and biomass on T.matsutake,and the potential effects of environmental variables on microorganism of T,matsutake shiroes.The microbe quantity of microorganisms(bacteria,actinobacteria,and fungi),the microbial biomass(microbial biomass carbon and nitrogen),and the enzyme activities(acid phosphatase,urease,and catalase) were analyzed.Multivariate regression tree was used to study the microbial activity and microbial biomass associated with environmental variables including elevation,slope,thickness and coverage of litter.The results showed that above the 3592 m elevation,the enzyme activities of T.matsutake shiroes increased with the elevation(3841-3860 m);below the 3592 m elevation,however,the enzyme activities increased with the litter thickness(2.25-8.45 cm)but decreased with elevation(3058-3226 m).With slopes greater than 42.5°,the microbial quantity and biomass increased with the litter thickness(1.05-8.45 cm) and decreased with elevation(3058-3225 m);with slopes smaller than 42.5°,however,the microbial quantity and biomass decreased with both litter thickness(0.28-3.2 cm) and elevation(3058-3460 m).In conclusion,elevation,slope,and litter thickness are the major environmental factors affecting the soil microbial activity and biomass in T.matsutake shiroes.更多还原