【摘要】 目的测定桃金娘根中没食子酸和总多酚的含量。方法没食子酸的含量测定采用超高效液相色谱法。色谱条件:以甲醇-0.5%磷酸(15:85)为流动相,采用二级管阵列检测器(PDA)检测,检测波长为215 nm,流速为0.2 mL/min。总多酚的含量测定采用分光光度法,以没食子酸为对照品,福林酚试剂为显色剂,在748 nm波长下进行测定。结果没食子酸在3.853~115.6μg/L(r=0.9999)浓度范围内与峰面积呈良好的线性关系,回收率为98.73%~100.76%。总多酚分析的线性范围为1.0~7.0μg/L(r=0.9995),回收率为98.04%~101.17%。结论该方法简便、快速、重现性好,可用于桃金娘根的质量控制及定量分析。更多还原

【Abstract】 Objective To determine the content of gallic acid and total polyphenol in Radix Rhodomyrti.Methods Gallic acid in Radix Rhodomyrti was determined by ultra performance liquid chromatography(UPLC) with photo-diode-array(PDA) at 215 nm,and the mobile phase was methol-0.5% phosphate acid solustion(15:85) at a flow rate of 0.2 mL/min.The content of total polyphenol was analyzed by spectrophotometry at 748 nm,gallic acid was selected as the reference substance.The colour-developing agent was Folin-Ciocalteu’s phenol reagent.Results The linear range for gallic acid and total polyphenol was 3.853-115.6 μg/mL(r = 0.9999) and 1.0-7.0 μg/mL(r = 0.9995) respectively.The recovery rate of gallic acid was 98.73% -100.76% and recovery rate of total polyphenol was 98.04% -101.17% .Conclusion The method is simple,rapid and good reproducible,can be used for quality control and quantitative analysis of Radix Rhodomyrti.更多还原