【摘要】 目的研究鼠卵胞浆内单精子显微注射(ICSI)改良技术在人类辅助生殖技术应用中的价值。方法将小鼠分为两组:采用固定人卵子的固定针组(C-ICSI组)和改良的喇叭型固定针组(N-ICSI组),观察两组ICSI操作后卵子存活数、卵裂情况。结果 N-ICSI组卵子存活率为77.4%,明显高于C-ICSI组的7.8%,差异有高度统计学意义(χ2=483.183,P<0.01);两组正常受精率及异常受精率比较,差异均无统计学意义(P>0.05)。N-ICSI组卵裂数为97.3%,明显高于C-ICSI组的80.0%,差异有高度统计学意义(χ2=18.399,P<0.01),桑葚胚、囊胚和孵化的胚胎比例差异无统计学意义(P>0.05)。结论改良的小鼠ICSI技术,可以明显提高操作时卵子的存活率,这将为人类实验室ICSI技术的质量控制和研究提供保障,同时,该项研究成果可以分析人精子激活卵母细胞的能力和人精子的核型,因此有重要的研究价值。更多还原

【Abstract】 Objective To study the value of improved technology ICSI in the mouse in the human assisted reproductive technology. Methods The ICSI operation were divided into two groups: ICSI operation with holding pipette for holding human ova(C-ICSI group) and with improved holding pipette(N-ICSI group), ova survival and cleavage in the two groups were observed after ICSI operation. Results The rate of ova survival in the N-ICSI group was 77.4%, significantly higher than that in the C-ICSI group(7.8%), there was statistically significant(χ2=483.183, P < 0.01). About the normal fertilization rate and abnormal fertilization rate, there was no significant difference(P > 0.05). The number of cleavage in the N-ICSI group was 97.3%, significantly higher than that in the C-ICSI group(80.0%), there was statistically significant difference(χ2=18.399, P < 0.01). There was no significant difference about the morula, blastocyst and hatching embryo(P > 0.05). Conclusion The modified ICSI technique in the mouse can significantly improve the survival rate of ova, which will provide the guarantee for quality control and research of human laboratory of ICSI technology, at the same time, the research can analyze the ability of human sperm for oocyte activation and karyotype analysis of human sperm, therefore there is an important research value.更多还原