【摘要】 结合笔者所在课题组的实验研究成果,综述人血清白蛋白融合技术研究进展。人血清白蛋白融合技术是近些年来应用较多的用于改造小分子蛋白和多肽药物的手段。但是众多的研究结果发现将小分子蛋白/多肽和人血清白蛋白融合后会出现一些共性的问题,例如目标蛋白产率低、易降解等。这些共性问题可以通过融合蛋白的优化设计、蛋白酶缺陷宿主的构建、融合基因多拷贝以及与分子伴侣共表达得以解决。更多还原

【Abstract】 The research progress in fusion technique of human serum albumin(HSA) was reviewed in this paper.Nowadays HSA fusion technique is a common and effective method for reforming small-molecule protein or peptide drugs.Many studies have shown that there are some common problems in the fusion of small molecule proteins or peptides with HSA,such as low yield and degradation of the target protein.However,these problems can be addressed by optimized design of the fusion protein,construction of protease-defi cient hosts,multiple copies of fused genes and coexpression with chaperones.更多还原