【摘要】 目的:观察丙泊酚麻醉对成年大鼠空间认知功能及海马CA1区GSK-3β水平的影响。方法:成年SD大鼠经尾静脉注射丙泊酚麻醉6 h,苏醒后24 h接受莫里斯水迷宫(Morris Water Maze,MWM)训练,观察全身麻醉对大鼠认知功能的影响;采用免疫荧光和Western blot技术,检测大鼠海马CA1区GSK-3β水平的变化。结果:与对照组相比,丙泊酚麻醉组大鼠在MWM任务的隐藏平台(P<0.01)、反向平台(P<0.01)和空间探索实验(P<0.05)的空间学习和记忆能力显著下降;同时伴有CA1区磷酸化的GSK-3β(p-GSK-3β)表达水平的下降(P<0.05),GSK-3β水平不变(P>0.05)。结论:丙泊酚麻醉可引起成年大鼠空间学习和记忆功能损害,其机制可能与降低海马CA1区的GSK-3β的磷酸化水平有关。更多还原

【Abstract】 Objective: To investigate whether propofol impairs the spatial learning and memory abilities and alters the level of GSK- 3β( glycogen synthase kinase- 3beta,GSK- 3β) in the CA1 of hippocampus in adult rats.Methods: Rats received propofol anesthesia via intravenous injection for 6 h. Moriss Water Maze training was conducted 24 h after the end of anesthesia to observe the effect of propofol anesthesia on learning and memory function in SD adult rats,and the level of GSK- 3β in the CA1 in rats was detected by Western blot and immunofluorescence.Results: Propofol anesthesia impaired learning and memory associated with hidden platform and reversal platform training,as well as probe test in adult rats. Moreover,the level of p- GSK- 3β in the CA1 of rats decreased significantly after propofol treatment.Conclusion: Propofol anesthesia induces spatial learning and memory impairment in adult rats,which may be associated with the decrease of p- GSK- 3β in the CA1.更多还原