【摘要】 目的:建立固相萃取(SPE)净化后紫外分光光度法(UV)快速测定黄花蒿中青蒿素含量。方法:黄花蒿干叶粉末中的青蒿素用无水乙醇超声波提取(200 W,40 k Hz),最佳提取条件为料液比1∶20(g∶m L),超声波萃时间15 min,温度25℃。提取液0.05 mol·L-1氢氧化钠水溶液45℃衍生20 min。衍生化后经C18SPE柱净化,乙醇-0.01 mol·L-1磷酸盐缓冲液(p H 5.8)(15∶85)洗涤杂质,乙醇-0.01 mol·L-1磷酸盐缓冲液(p H5.8)(33∶67)洗脱青蒿素衍生物Q259。洗脱液用紫外分光光度计259 nm波长处测定。结果:经高效液相色谱(HPLC)检测,SPE净化后Q259回收率在98.0%~105.9%;Q259对λ259光吸收贡献从26.8%~53.5%提高到92.0%~100%,λ259有光吸收的杂质基本除去;SPE-UV法与HPLC法相对误差小于10%。结论:SPE-UV法经方法学验证,可作为黄花蒿种植、收购时大量、快速检测黄花蒿中青蒿素含量的检测方法。更多还原

【Abstract】 Objective: To establish a method for rapid determination of artemisinin in Artemisia annua L. using solid phase extraction( SPE) followed by UV-spectrophotometry. Methods: Artemisinin was extracted from A. annua using anhydrous ethanol as the extracting solvent by ultrasonic wave( 200 W,40 k Hz). The optimal extraction condition was as follows: the solid-liquid ratio 1∶ 20( g∶ m L),the extraction time 15 minutes at 25 ℃. The extraction solution was derivatized in 0. 05 mol·L-1Na OH solution for 20 min at 45 ℃. The sample was purified by C18 SPE column with ethanol-0. 01 mol·L-1phosphate buffer( p H5. 8)( 15∶ 85),eluted by ethanol-0. 01 mol·L-1phosphate buffer( p H 5. 8)( 33∶ 67) and detected by UV-pectrophotometry at 259 nm. Results: The recovery rate of Q259 was between 98. 0% and 105. 9% after purifying by SPE which was detected by HPLC. And the contribution of Q259 for light absorption in λ259improved from 26. 8%-53. 5% to 92. 0%-100%,which indicated that the impurities absorbed in λ259were almost removed. The relative error between SPE-UV and HPLC was less than 10%.Conclusion: The SPE-UV after verification methodology could work effectively in on-site rapid determination of arteannuin content in large quantity of A. annua during planting and purchasing.更多还原