Objective:To develop a method for the content determination of dihydromyricetin(DMY)and myricetin(MY)in leaves of Ampelopsis grossedentata.Methods:HPLC method was adopted.The separation was performed on SUPELCO Discovery C18(25cm×4.6mm,5μm)column with 0.03% phorsphoric acid-methanol as mobile phase at flow rate of 0.8mL·min-1.The detection wavelength of MY was set at 373 nm,and that of DMY was set at 290 nm.Results:The linear range was 0.15~94mg·L-1(R2=1)for DMY and 0.62~77.50mg·L-1(R2=0.999 7)for MY with a...