【摘要】 利用7个SSR位点对沈阳动物园丹顶鹤核心种群进行了遗传多样性分析,统计了等位基因数（N_a）、有效等位基因数（N_c）,计算了群体杂合度（H_e）、多态信息含量（PIC）、Shannon信息指数（I）、近交系数(F_is)等参数。结果显示:7个微卫星标记中有4个高度多态位点和3个中度多态位点,共扩增出36个等位基因,并发现了11个稀有等位基因;平均有效等位基因是2.4342,平均期望杂合度为0.5510,平均多态信息含量是0.5049,遗传多样性水平较高;哈温平衡检测显示5个位点符合哈温平衡,2个位点偏离了哈温平衡;平均F_is值为0.0069,同时平均观测杂合度小于平均期望杂合度,显示可能存在种群个体亲缘关系较近的情况。更多还原

【Abstract】 We analyzed the genetic diversity of the red-crowned crane（Grus japonensis） population at Shenyang Forest Zoo by using 7 SSR loci.We counted numbers of alleles（N_a）and effective numbers of alleles（N_e）.and calculated polymorphism information content（PIC）.Shannon information index（I） and inbreeding coefficient(F_is).There were 4 highly polymorphic loci and 3moderately polymorphic loci.36 alleles were amplified,and 11 rare alleles were found.The average N_ewas 2.4342.the average expected heterozygosity was 0.5510.and the average PIC was0.5049.showing higher levels of genetic diversity.Hardy- Weinberg equilibrium | HWE) testing showed that 5 SSR loci were in accord with HWE and 2 loci were not in HWE.Mean Fis was0.0069,while the average expected heterozygosity was greater than the average observed heterozygosity,indicating that there were closer relationships between some individuals.更多还原