【摘要】 目的:建立同时分析测定芎菊上清片中4个活性成分（α-松油醇、薄荷酮、薄荷脑和胡薄荷酮）的方法。方法:采用GC法,色谱柱为DB-1701毛细管色谱柱（30 m×0.3 mm×0.25μm）,柱温采用程序升温(80℃保持1 min,以5℃·min^-1的速率升温至120℃,保持5 min,再以50℃·min^-1的速率升温至220℃,保持2 min),进样口温度200℃,FID检测器,检测器温度230℃。结果:α-松油醇、薄荷酮、薄荷脑和胡薄荷酮线性范围分别为0.01⁰.55 mg·mL^-1（r=0.999 9）、0.10⁰.50 mg·mL^-1（r=0.999 8）、0.01⁰.50 mg·mL^-1（r=0.999 8）、0.01⁰.51 mg·mL^-1（r=0.999 9）;平均加样回收率（n=6）分别为97.6%、98.2%、95.7%、96.1%,其RSD分别为1.5%、1.4%、1.6%、1.6%。结论:该方法操作简单,重复性好,可有效控制芎菊上清片的质量。更多还原

【Abstract】 Objective:To establish a method for simultaneous determination of four active ingredients（α- terpineol,menthone,menthol,pulegone） in Xiongjushangqing Tablets.Methods:GC method was adopted.The determination was performed on a DB-1701 column（30 m ×0.25 mm ×0.25 μm）.The column temperature was programmed(maintained at 80 ℃ for 1 min,then risen to 120 ℃ at the rate of 5 ℃ ? min^-1,held for 5 min.After that the temperature was elevated to 220 ℃ at the rate of50 ℃ ? min^-1 and held for 2 min).The FID was used as detector,the temperatures of the injection and the detector were set at200 ℃ and 230 ℃.Results;The calibration curves were linear in the ranges of 0.01-0.55 mg ? mL^-1 for α- terpineol（r =0.999 9）,0.10-0.50 mg ? mL^-1 for menthone（r = 0.999 8）,0.01- 0.50 mg ? mL^-1 for menthol（r = 0.999 8） and0.01-0.51 mg ? mL^-1 for pulegone（r = 0.9999）.The average recoveries（n=6） were 97.6%,98.2%,95.7%and96.1%.RSD were 1.5%,1.4%,1.6%and 1.6%,respectively.Conclusion;The method is simple and repeatable,which can be used for quality control of Xiongjushangqing Tablets.更多还原