【摘要】 本研究以抗菌肽Gloverins为研究对象,分析其抗性机理,并根据其编码区序列建立系统发育树,分析Gloverins的进化及结合生物信息学软件分析初步探索抗菌差异性产生的原因。结果表明:Gloverins与细菌或真菌表面的LPS,LTA,PG及昆布多糖相互作用是有效对抗微生物的必要条件。Gloverins的基因序列有一定的保守性,其中,玉米穗虫和棉铃虫同源性最高为98.4%,亲缘关系较近。家蚕中有4个完整的Gloverins基因,其中,Bm Glv1为其他3种同系物的祖先基因。大部分Gloverins对革兰阴性菌具有极强的杀灭作用,而甜菜夜蛾Gloverin(Se Glv)及烟草天蛾Gloverin(Ms Glv)由于其与Toll基因表达的依赖性,与LPS,LTA,PG和昆布多糖的结合位置,α-螺旋比例以及某些关键位点的氨基酸等因素,导致其对革兰氏阳性菌有抗菌活性,而对革兰阴性菌无活性的抗菌特性。更多还原

【Abstract】 Based on antimicrobial peptides Gloverins gene,we analyzed the resistance mechanism,and established the system development tree and discussed the antibacterial peptide origins,evolution and the cause of the antibacterial differences. The results showed that the interaction between antibacterial peptides and LPS,LTA,PG and laminarin on the surface of bacteria or fungi was the necessary condition to effective defend microbes. Gloverins gene was conservative; the homology of Helicoverpa zea and Helicoverpa armigera was the highest( 98. 4%). Silkworm has four complete antibacterial peptide genes,and Bm Glv1 is ancestor gene of the other three homologues. Most of the antimicrobial peptides have a strong exterminate action on gram-negative bacteria,but due to rely on the Toll gene expression,the combination position with LPS,LTA,PG and laminarin α-spiral than columns,and some key sites of amino acids and other factors,Spodpptera exigua and Manduca sexta Gloverins have antimicrobial activity on the gram positive bacteria,but inactive to gram-negative bacterium antibacterial properties.更多还原