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玉米生物诱导孤雌生殖DH群体的SNP偏分离分析

Segregation Distortion study on Bio-induced Parthenogenesis-derived Doubled Haploid Lines of Maize by SNP

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【作者】 许理文段民孝宋伟田红丽王凤格赵久然王守才

【Author】 XU Li-wen;DUAN Min-xiao;SONG Wei;TIAN Hong-li;WANG Feng-ge;ZHAO Jiu-ran;WANG Shou-cai;College of Agriculture and Biotechnology, China Agricultural University;Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences;

【机构】 中国农业大学农学与生物技术学院北京市农林科学院玉米研究中心

【摘要】 以单交种先玉335作母本,与京科诱006单倍体诱导系杂交,产生单倍体及秋水仙素加倍形成双单倍体(DH)群体,用SNP芯片对DH群体进行基因型分析,获得1 337个在先玉335的双亲间有多态的SNP数据,对SNP进行卡方检测(P<0.05),发现516个SNP表现偏分离,占38.6%,这些偏分离SNP中有285个偏向母本PH6WC,占55.2%;231个偏向父本PH4CV,占44.8%。在玉米的8条染色体上,发现10个偏分离热点区域(SDR),分析SNP的χ2值与其在染色体上的位置之间的关系发现,χ2值最大的SNP往往在SDR的中心位置。

【Abstract】 Haploids originating from the hybrid, Xianyu335(PH6WC×PH4CV) were crossed with the haploid in-ducer, Jingkeyou006 and the resulting doubled haploid lines(DHs) were obtained by chromosome doubling with col-chicine. A total of 1337 SNP markers detected polymorphism between PH6 WC and PH4 CV. Among 1 337 SNP, cov-ering whole maize 10 chromosomes, five hundred and sixteen SNP(38.6%) showed the genetic distortion(P<0.05). Ofthe total segregation distortion SNP, two hundred and eighty- five SNP(55.2%) deviated toward female parent,PH6 WC, while 231 SNP(44.8%) deviated toward male parent, PH4 CV. Totally, tensegregation distortion regions(SDRs) were detected among 8 different chromosomes, the relationship between χ2valueand position in chromosomeof SNP was analyzed.

【关键词】 玉米SNP偏分离加倍单倍体
【Key words】 MaizeSNPSegregation distortionDoubled haploid(DH)
【基金】 北京市科技计划课题(D131100000213002);国家科技支撑项目(2011BAD35B09);北京市农林科学院科技创新能力建设专项(KJCX20140202);“863”计划“玉米产量功能基因研究与克隆”(2012AA10A305)
  • 【文献出处】 玉米科学 ,Journal of Maize Sciences , 编辑部邮箱 ,2015年03期
  • 【分类号】S513
  • 【被引频次】8
  • 【下载频次】245
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