【摘要】 目的建立T细胞依赖性抗体反应(TDAR)大鼠模型,研究参麦注射液(SMIJ)对大鼠免疫功能的影响。方法 SD大鼠按分组分别ig给予环孢素A(CSA)0.02 g·kg-1,iv给予SMIJ溶剂或SMIJ 0.6,1.2和2.4 g·kg-1,每天1次,连续28 d。于给药第15天和第22天经左后足趾sc给予钥孔其虫戚血蓝蛋白(KLH)2.4 mg·kg-1进行免疫刺激。实验期间观察大鼠的一般状态,每周测体质量和摄食量。在第20天和第29天用ELISA法分别测定大鼠血清KLH-Ig M和KLH-Ig G抗体浓度,第29天活杀大鼠,应用全自动血液分析仪测定血液红细胞和白细胞数及白细胞分类,用天平称取肺、肝、脾和胸腺质量并计算脏器系数,采用HE染色法观察肺、肝、脾、胸腺和淋巴结组织病理变化。结果实验期间未见大鼠死亡,各给药组体质量增长和摄食量无异常。与正常对照组相比,模型组大鼠血清KLH-Ig M和KLH-Ig G抗体浓度均明显增高(P<0.01),提示KLH引起机体免疫应答;与模型组相比,血清KLH-Ig M和KLH-Ig G抗体浓度在CSA组均明显降低(P<0.01),而在SMIJ溶剂组和给药组则无明显变化。给药后第29天,与正常对照组相比,模型组大鼠各项血液学指标和脏器系数无差异,各组织脏器亦无明显病变;与模型组相比,CSA组白细胞、嗜酸性粒细胞和淋巴细胞数、脾和胸腺脏器系数均明显降低(P<0.05,P<0.01),脾出现白髓范围和脾小体淋巴细胞数目减少及淋巴小结界限不清等免疫抑制现象,而SMIJ溶剂和给药组各项血液学和脏器系数均无明显改变,各组织脏器亦无明显病变。结论建立了SD大鼠TDAR研究模型,连续尾静脉给予SMIJ 28 d对SD大鼠TDAR无明显影响。更多还原

【Abstract】 OBJECTIVE To study the effect of Shenmai injection( SMIJ) on immune function in rats by continuous iv administration by establishing a T cell-dependent antibody response( TDAR) model.METHODS SPF SD rats were ig given ciclosporin A( CSA) 0. 02 g·kg- 1or iv given SMIJ solvent and SMIJ 0. 6,1. 2 and 2. 4 g·kg- 1,once daily for 28 d. On the 15 th and 22 nd days,each rat except normal control group was sc given keyhole limpet hemocyanin( KLH) 2. 4 mg·kg- 1. The general state of the rats was observed throughout the trial while body mass and food consumption were measured weekly.The concentration of serum specific KLH-Ig M antibody of rats was detected on the 20 th day while the cencentration of serum specific KLH-Ig G antibody of rats was detected on the 29 th day,when all rats were sacrificed,and the number of red blood cells,white blood cells and the classification of white blood cells were detected by automatic blood analyzer. The mass of the lung,liver,spleen and thymus was weighed by a scale,organ indexes were calculated,and the histopathological changes of the lung,liver,spleen,thymus and lymph nodes were observed by HE staining. RESULTS There was no death or significant difference in body mass and food consumption of rats between these groups during the experiment. The concentration of serum specific KLH-Ig M and KLH-Ig G antibody in model group was higher than in normal control group( P < 0. 01),suggesting that KLH had caused immune response. When compared with the model group,the concentration of serum KLH-Ig M and KLH-Ig G antibody was significantly reduced in CSA group( P < 0. 01) while there was no significant change in SMIJ dose and SMIJ solvent groups. On the 29 th day,the hematologic and organ index showed no difference and the organs had no lesions in the model group when compared with the normal control group. When compared with the model group,the number of white blood cells,eosinophils and lymphocytes and spleen and thymus organ indexes was significantly reduced in CSA group( P < 0. 05,P < 0. 01),the scope of splenic white pulp and the number of splenic lymphocytes were reduced and the boundary of lymphoid nodules was clear in CSA group,the results of hematology and organ indexes were not significantly changed and the organs had no obvious lesions in SMIJ and SMIJ solvent groups. CONCLUSION The TDAR model in SD rats is successfully established. Continuous iv injection of SMIJ for 28 d has no obvious effects on TDAR in SD rats.更多还原