【摘要】 目的观察小干扰RNA(siRNA)靶向下调高迁移率族蛋白A2(HMGA2)基因表达对人前列腺癌DU145细胞增殖和凋亡能力的影响。方法采用脂质体LipofectamineTM2000将HMGA2-siRNA(siRNA组)和阴性对照siRNA(阴性对照组)分别转染至DU145细胞中;空白组无特殊处理。采用RT-PCR及Western blot法分别检测HMGA2 mRNA和蛋白的表达;细胞增殖-毒性检测(CCK-8)法检测DU145细胞的增殖;流式细胞术检测细胞凋亡。结果 siRNA组HMGA2mRNA的相对表达量为0.562±0.067,低于阴性对照组的0.858±0.043和空白组的0.876±0.041(P<0.05)。siRNA组细胞的增殖能力也低于其他两组(P<0.05)。转染后48h,siRNA组DU145细胞的凋亡率为(14.180±0.395)%,高于阴性对照组的(8.267±0.379)%和空白组的(7.513±0.476)%(P<0.05)。结论 HMGA2基因特异性siRNA可抑制DU145细胞增殖,促进细胞凋亡。更多还原

【Abstract】 Objective To study the effect of high mobility group A2(HMGA2)down-regulation by small interference RNA(siRNA)on the proliferation and apoptosis of the prostate cancer cell line DU145.Methods With LipofectamineTM2000,HMGA2-siRNA(group A)and negative control-siRNA(group B)were transfected into DU145 cells and then the expressions of HMGA2 mRNA and protein were detected by RT-PCR and Western blot,respectively.The proliferation and apoptosis of DU145 cells were detected by cell counting kit-8(CCK-8)and flow cytometry,respectively.The results were compared to those of blank controls(group C).Results The expression of HMGA2 mRNA in group A was 0.562±0.067,which was lower than 0.858±0.043 in group B and 0.876±0.041 in group C.So did the cell proliferation(P<0.05).The apoptosis rate of DU145 cells in group A was(14.180±0.395)%,which was higher than(7.513±0.476)%in group B and(8.267± 0.379)%in group C(P<0.05).Conclusion Silencing HMGA2 gene can effectively inhibit the expressions of HMGA2 mRNA and protein,decrease the ability of proliferation,and promote the apoptosis of DU145 cells.更多还原