【摘要】 目的探讨受体O型蛋白酪氨酸磷酸酶(PTPRO)对妊娠期肝内胆汁淤积症(ICP)患者Th1/Th2细胞偏倚的影响。方法分离获取正常妊娠(A组,30例)、轻型ICP(B组,28例)和重型ICP(C组,30例)孕妇胎盘来源的单核-巨噬细胞,采用RT-PCR和Western blot法分别检测PTPRO mRNA和蛋白的表达,Western blot法检测NF-κB/p65的磷酸化水平。分离获取孕妇外周血T淋巴细胞,与胎盘来源的单核-巨噬细胞共培养,ELISA法检测细胞培养上清液中IFN-γ、IL-12、IL-4和IL-10水平。结果与A组相比,B、C组单核-巨噬细胞PTPRO表达和NF-κB/p65磷酸化水平升高,与其共培养的外周血T淋巴细胞高分泌IFN-γ和IL-12(P<0.05);与B组相比,C组PTPRO表达和NF-κB/p65磷酸化水平升高,IFN-γ和IL-12分泌增多(P<0.05)。结论 ICP患者胎盘单核-巨噬细胞高表达PTPRO,促使T淋巴细胞向Th1细胞偏倚。更多还原

【Abstract】 Objective To explore the effects of protein tyrosine phosphatase receptor-type O(PTPRO)on Th1/Th2 bias in pregnant women with intraheptic cholestasis(ICP).Methods Monocytemacrophages were separated from placental tissues of pregnant women with normal term(group A,30 cases),mild ICP(group B,28 cases)and severe ICP(group C,30 cases).The expressions of PTPRO protein and mRNA and the level of phosphorylated NF-κB/p65 were detected by RT-PCR and Western blot,respectively.The T lymphocytes of eripheral blood and monocyte-macrophages separated from placental tissues were co-cultured and the expressions of IFN-γ,IL-12,IL-4 and IL-10 in supernatant were determined by ELISA.Results The expression of PTPRO and the phosphorylation level of NF-κB/p65 in monocyte-macrophages were higher and the secretions of IFN-γand IL-12 from T lymphocytes were more in groups of B and C than those in group A(P<0.05),which were more in group C than those in group B(P<0.05).Conclusion PTPRO is highly expressed in placental monocyte-macrophages of pregnant women with ICP,which promotes the bias of T lymphocytes to Th1 cells.更多还原