节点文献

血小板的软X射线谱学显微成像研究

Microspectroscopic soft X-ray imaging analysis of platelet

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 周铭唐海沁蒋诗平张勇

【Author】 Zhou Ming;Tang Haiqin;Jiang Shiping;Dept of Cadre Cardiovascular,The First Affiliated Hospital of Anhui Medical University;National Synchrotron Radiation Laboratory of China University of Science and Technology;

【机构】 安徽医科大学第一附属医院老年心内科中国科学技术大学国家同步辐射实验室

【摘要】 探索性地应用软X射线谱学显微技术研究血小板的结构及钙元素成像的变化,采用谱学显微术对制取的SD大鼠血小板形态扫描透射成像并对血小板作钙元素双能对比衬度成像。血小板成像可看到血小板大小不等,有的伸出伪足;钙元素分布成像血小板在静息状态时胞内钙元素主要分布于质膜侧凹陷的钙池,诱导聚集后则扩散分布于胞质。软X射线谱学显微成像能反应血小板钙元素分布,为钙的探测提供一种新方法。

【Abstract】 To test the transformation of intracellular calcium in platelet initially applying microspectroscopy soft Xray for imaging based on the truth the platelets were at the status of activation in a variety of illness. Normal adult SD rats were enrolled in the trial and finally the rat platelets were extracted for platelet scanning transmission and dual-energy calcium contrast imaging. From the morphology and distribution of calcium of platelet,a various type of platelet morphology pictures were observed,of which the cell deformed and the parapodium stretched out; while calcium mainly distributed in the calcium pool near plasmalema under quiescent condition but scattered across the cytoplasm in activated condition induced by adenosine diphosphate. In conclusion,the microspectroscopy soft X-ray imaging was capable of reflecting the distribution of calcium in the platelet and provides a practicable new method for the measure of intracellular calcium.

【基金】 安徽省科技攻关项目(编号:12010402116)
  • 【文献出处】 安徽医科大学学报 ,Acta Universitatis Medicinalis Anhui , 编辑部邮箱 ,2015年06期
  • 【分类号】R816.3
  • 【下载频次】92
节点文献中: 

本文链接的文献网络图示:

本文的引文网络