【摘要】 目的探讨肿瘤转移抑制基因KAI1/CD82、细胞黏附分子CD44v6在人喉鳞状细胞癌(LSCC)、癌前病变、息肉以及正常黏膜中的表达。方法应用免疫组化法检测64例LSCC、21例喉癌前病变、15例喉息肉和15例正常喉黏膜组织中KAI1/CD82、CD44v6蛋白的表达水平。结果1KAI1/CD82蛋白在正常喉黏膜及喉息肉组织中呈高表达,喉癌前病变及LSCC组织中呈低表达,阳性表达率分别为86.67%(13/15)、80.00%(12/15)、38.10%(8/21)、37.50%(24/64),正常喉黏膜与LSCC两者之间比较差异有统计学意义(P<0.05)。2 CD44v6蛋白在正常喉黏膜及喉息肉中呈低表达,在喉癌前病变及LSCC组织中呈高表达,阳性表达率分别为26.67%(4/15)、33.33%(5/15)、80.95%(17/21)、75.00%(48/64),正常喉黏膜与LSCC两者之间比较差异有统计学意义(P<0.05)。结论 1 KAI1/CD82表达下调、缺失及CD44v6表达上调与LSCC的发生、发展相关。2联合检测KAI1/CD82和CD44v6可为LSCC的早期诊断提供临床依据。更多还原

【Abstract】 Objective To study the expression of metastasic suppressor gene( KAI1 / CD82) and cell adhesion molecules( CD44v6) in human laryngeal squamous cell carcinoma( LSCC),precancerous lesion,polyps,and normal mucosa. Methods Immunohistochemical technique( Envision) was used to detect the expression of KAI1 / CD82 protein and CD44v6 protein in 64 cases of LSCC,21 cases of laryngeal precancerous lesion( LPL),15 cases of polyp of larynx( LP) and 15 cases of normal laryngeal mucosa( NLM). Results The result turned out to be as follows:1KAI1 / CD82 protein was highly expressed in NLM and LP,and lowly expressed in LPL and LSCC,the positive rates of KAI1 / CD82 protein expression were 86. 67%(13 /15),80. 00%(12 /15),38. 10%(8 /21) and 37. 50%(24 /64),respectively. There was statistically significant difference in NLM and LSCC. 2 CD44v6 protein was lowly expressed in NLM and LP and highly expressed in LPL and LSCC,the positive rates of CD44v6 protein expression were 26. 67%(4 /15),33. 33%(5 /15),80. 95%(17 /21) and 75. 00%(48 /64),respectively. There was statistically significant difference in NLM and LSCC. Conclusion 1 The down-regulation or deletion of KAI1 / CD82 and the up-regulation of CD44v6 are related to carcinogenesis,development of LSCC. 2 The combined detection of KAI1 / CD82 and CD44v6 may provide clinical basis for the early diagnosis of LSCC.更多还原