【摘要】 目的探讨受体相互作用蛋白1(RIP1)增强顺铂(DDP)诱导食管癌细胞凋亡的敏感性。方法单溶液细胞增殖分析(MTS)法检测不同浓度DDP对食管癌细胞株KYSE510、KYSE410的增殖抑制作用;Annexin V/PI双染流式细胞术检测细胞凋亡;Western blot法检测RIP1、半胱天冬氨酸蛋白酶3(caspase-3)、PARP的蛋白表达。结果 DDP诱导食管癌细胞凋亡具有剂量和时间相关性。凋亡率随剂量增加和时间延长升高,RIP1蛋白的表达升高,DDP联合RIP1特异性抑制剂处理食管癌细胞后,较敏感的KYSE510凋亡率明显减少。结论 RIP1可能参与了DDP诱导食管癌细胞凋亡的作用。更多还原

【Abstract】 Objective To investigate the role of receptor-interacting protein 1( RIP1) on the sensitivity of human esophageal squamous carcinoma cells to cisplatin( DDP)-induced apoptosis and explore a new target for clinical treatment of esophageal squamous carcinoma. Methods The viability of human esophageal squamous carcinoma cell lines KYSE510 and KYSE410 exposed to different concentrations of DDP were detected by Cell Titer 96AQueous One Solution Cell Proliferation Assay( MTS) assay. Annexin V / PI staining was used to observe cell apoptosis in KYSE510 and KYSE410 cells. Western blot was used to detect RIP1,caspase-3,PARP expression in KYSE510 and KYSE410 cells exposed to DDP. Results DDP induced apoptosis in esophageal squamous carcinoma cells with dose and time dependency. Apoptotic rate was increased with dose and time,RIP1 expression was upregulated in esophageal squamous carcinoma cell lines. It was significantly reduced after exposure to DDP association special inhibitor of RIP1 in KYSE510 cells which were more sensitive to DDP than KYSE410 cells. Conclusion RIP1 maybe participates in the apoptosis of human esophageal squamous carcinoma cells to DDP,suggesting the potential of RIP1 as a new candidate target for clinical treatment of esophageal squamous carcinoma.更多还原