【摘要】 目的评估2种育发剂对中国仓鼠肺细胞(CHL)细胞活力的影响及其诱导染色体畸变的能力。方法在非代谢活化和代谢活化情况下,采用MTT法和体外哺乳动物细胞染色体畸变试验检测2种(编号A和B)育发剂对CHL细胞活力和染色体畸变率的影响。结果 A育发香波在不含S9的系统中,受试物终浓度为0.156μl/ml时细胞存活率为54.9%;在含S9的系统中,受试物终浓度为0.313μl/ml时细胞存活率为52.4%;B育发膏在不含或含S9的系统中,受试物终浓度为5 mg/ml时细胞存活率均超过90%。与对照组比较,A育发香波各剂量组的染色体畸变率差异无统计学意义(不加S9的体系下Pearson Chi-Square值=0.341,P=0.952,在S9体系下的Pearson Chi-Square值=0.687,P=0.876),B育发膏在加S9和不加S9试验体系下高剂量组的染色体畸变率与阴性对照组相比,有显著性差异(不加S9的体系下Pearson Chi-Square值=16.611,P=0.000,在S9体系下的Pearson Chi-Square值=11.971,P=0.01;重复试验不加S9的体系下Pearson Chi-Square值=15.457,P=0.000,在S9体系下的Pearson Chi-Square值=15.341,P=0.000),低、中剂量组的染色体畸变率无显著性差异(不加S9的体系下Pearson Chi-Square值=0.000,P=1.000,在S9体系下的Pearson Chi-Square值=0.687,P=0.709)。结论在本实验条件下,A育发香波无致突变性,B育发膏有致突变性。更多还原

【Abstract】 Objective To evaluate the influence of two kinds of hair restorer on cell viability and chromosomal aberrations of Chinese hamster lung( CHL) cells. Methods Cell viability was assessed by MTT assay and chromosome damage was detected by in vitro mammalian cells chromosome aberration test in the presence and absence of the liver homogenate fraction( S9 mix). Results Cell viability treated with A Shampoo was 54. 9%when A shampoo at 0. 15625 u L / mL in the absence of S9 mix, while it was 52. 4% when A Shampoo at0. 3125 u L / mL in the presence of S9 mix. Cell viability treated with B ointment was over than 90% when B ointment at 5 mg / mL both in the presence and absence of S9 mix. Chromosomal aberration rate in A Shampoo treated group showed no statistical significance with H2 O negative control group with or without S9 mix( Without S9 mix: Pearson Chi- Square value = 0. 341,P = 0. 952,With S9 mix: Pearson Chi- Square value =0. 687,P = 0. 876). Chromosomal aberration rate in high- dosage B ointment treated group was significantlydifferent from solvent control group( without S9 mix:Pearson Chi- Square value = 16. 611,P = 0. 000,with S9 mix: Pearson Chi- Square value = 11. 971, P =0. 01; Repeat Test without S9 mix: Pearson Chi-Square value = 15. 457,P = 0. 000,with S9 mix: Pearson Chi- Square value = 15. 341,P = 0. 000). There were no significant differences in terms of chromosome aberration rate between low- dosage and middle- dosage B Ointment groups and solvent control group( without S9mix: Pearson Chi- Square value = 0. 000, P =1. 000,with S9 mix: Pearson Chi- Square value = 0. 687,P = 0. 709). Conclusion Under this condition,A shampoo shows no mutagenicity on CHL cell,while B ointment shows mutagenicity on chromosome aberration on CHL cells.更多还原